摘要
目的为了获得大量的、可供实验研究和临床应用的人ｌｅｐｔｉｎ。方法采用基因工程技术，把肥胖基因（ｏｂｅｓｅｇｅｎｅ，ＯＢ）编码人ｌｅｐｔｉｎ的ｃＤＮＡ序列（ＯＢ１）定向插入高效原核细胞表达载体ｐＢＶ２２１，构建重组质粒ｐＢＶ２２１ＯＢ１，并转化大肠杆菌ＴＧ１。结果经过限制性内切酶分析和ＰＣＲ技术鉴定，重组质粒ｐＢＶ２２１ＯＢ１含有编码人ｌｅｐｔｉｎ的基因序列。
Objective To get human
leptin which can be used in experimental study and clinical application. Methods With gene
engineering technique, human leptin cDNA sequence (OB1) was inserted directly into pBV221, a
proeukaryotic cell expression vector, to construct recombinant pBV221OB1 and to transform E.
coli strain TG1. Results Through the analysis of restrictive endonuclease and polymerase
chain reaction (PCR), recombinant pBV221OB1 contained the cDNAsequencecodinghumanleptin.
Conclusion Proeukaryotic cell expression system containing the cDNA sequence of human
leptin gene was constructed.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1999年第3期172-174,共3页
Chinese Journal of Endocrinology and Metabolism
