摘要
目的探讨肿瘤坏死因子α（TNF－α）与创伤后脑水肿发病的关系。方法分别于自由落体和液压脑创伤后用放射免疫法测定血清和脑组织中TNF－α的含量；用干湿草法测定脑水含量；用核着酸二磷酸酶标记小胶质细胞。结果自由落体脑创伤后血清和脑组织中TNF－α含量明显升高；脑水含量显著增加；小胶质细胞由正常的分枝样转变成为阿米巴样。在死亡率相近的情况下，液压脑创伤后血清和脑组织中TNF-α含量无明显变化；在观察的时间点内无脑水肿改变。结论TNF－α与脑水肿的产生有相关性。
Objective To explore the relationship between tumor necrosis factor a (TNF- a)and brain edema .Methods Radioi mmunoassay was used to measure the TNF-a in sera and cerebral homogenates ,dry and wet method to assay the brain tissur water content,nucleotide diphosphatase staining to label the microglia cells after comtusion and fluid percussion injury ,Resuits After the contusion brain injury TNF - a sera and cerebral homogenates and brain water content increased significantly , microglia changed from normal ramified to amoeboid form .TNF - a in sera and cerebral homogenates brain water content and the morphology of microglia did not change significantly after the fluid percussion brain injury .Conchusion There is signifcant relationship between TNF - a and brain edema .
出处
《中国急救医学》
CAS
CSCD
北大核心
1999年第6期323-325,共3页
Chinese Journal of Critical Care Medicine
基金
卫生部资助!96-1-302
