摘要
目的:研究颗粒细胞丙二醛(MDA)、超氧化物歧化酶(SOD)水平及凋亡对IVF-ET结局的影响。方法:选取51名首次行IVF-ET的输卵管性因素不孕患者。用GnRH-a长方案超促排卵治疗,密度梯度离心法分离卵泡液中颗粒细胞,硫代巴比妥酸法测定MDA水平,化学发光法测定SOD水平,流式细胞仪法测定凋亡率。结果:妊娠组与未妊娠组相比有较低的MDA水平和凋亡率,较高的优胚率,差异有统计学意义(P<0.05)。MDA与受精率呈中度负相关(r=-0.425,P=0.002),与获卵数、成熟卵子数、卵裂率、优胚率无明显相关关系;SOD与之均无相关关系;凋亡率与获卵数(r=-286,P=0.042)、成熟卵子数(r=-0.330,P=0.020)、优胚率(r=-0.311,P=0.026)呈低度负相关。MDA与SOD呈中度负相关(r=-0.471,P<0.001);与凋亡率呈中度正相关(r=0.475,P<0.001)。结论:氧化应激可能导致卵巢颗粒细胞凋亡,进而影响卵子的发育潜能及IVF-ET的结局。
Objective To examine the association among malondialdehyde（MDA）,superoxide dismutase（SOD） and incidence of apoptosis of granulosa cells in follicular fluid with the outcome of in vitro fertilization-embryo transfer（IVF-ET）.Methods We recruited 51 women undergoing an IVF-ET programme for tubal factor infertility.The women with endometriosis or endocrine diseases and those with male factor infertility were excluded.All the 51 patients underwent a long gonadotropin-releasing hormone（GnRH） agonist protocol for pituitary downregulation followed by controlled ovarian hyperstimulation with rFSH.Granulosa cells were isolated from all aspirated follicular fluid using gradient centrifugation at oocyte retrieval.The level of MDA and the activity of the SOD were measured by the thiobarbituric acid（TBA）and the chemiluminescence method,respectively.The apoptosis was studied by flow cytometry using propidium iodide.Results Twenty-six out of the 51 patients（51.0%） were pregnant after IVF-ET.Non-pregnant patients showed significantly higher MDA level [（1.7±0.72）nmol/（g·prot） vs.（1.1±0.56） nmol/（g·prot）,P〈0.05）],higher incidence of apoptosis（24.8%±6.57% vs.19.0%±5.59%,P〈0.05） and lower SOD level [（3.5±1.08）×10^3NU/（g·prot） vs.（4.4±0.99）×10^3NU/（g·prot）,P〈0.05）] in the granulose cells and lower good-embryo rate（54.9±20.22% vs.65.9±16.16%,P〈0.05） compared with the pregnant patients.No correlation was detected among SOD and the number of retrieved oocytes,oocyte maturity,embryo quality,fertilization,or cleavage.A significant negative correlation was detected between MDA and fertilization rate（r=-0.425,P=0.002）.No significant correlation was detected between MDA and age,the number of retrieved oocytes,oocyte maturity,cleavage,or good-embryo rate.A significant negative correlation was detected between the incidence of apoptosis and the number of retrieved oocytes（r=-0.286,P=0.042）,mature oocytes（r=-0.330,P=0.020）and good-embryo rate（r=-0.311,P=0.026）.There was significant negative correlation between MDA and SOD levels（r=-0.471,P〈0.001）;and significant positive correlation between MDA level and incidence of apoptosis（r=0.475,P〈0.001）.Conclusion Oxidative stress may induce apoptosis in granulose cells and subsequently lower oocyte quality and lead to poor outcome of IVF-ET.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2010年第9期990-994,共5页
Journal of Central South University ：Medical Science
关键词
颗粒细胞
氧化应激
凋亡
卵子质量
granulose cell
oxidative stress
apoptosis
oocyte quality
