摘要
目的 针对传统感染性结石造模方法的缺陷,设计一种新的改良的感染性膀胱结石动物模型.方法 将雄性SD大鼠24只分为模型组（n=12）、假手术组（n=8）和对照组（n=4）.模型组应用25%乌尔坦1.0 g/kg腹腔麻醉,下腹正中切开腹腔,提起膀胱,使用无菌G18穿刺针穿刺膀胱,经穿刺针将含菌异物推人膀胱;假手术组大鼠膀胱内不置异物,仅行无菌穿刺;对照组大鼠膀胱内不置入任何异物.手术后第21天,各组大鼠以过量麻醉方法全部处死,观察结石形成的情况.结果 造模术的第2天模型组大鼠死亡2只,其他各组无大鼠死亡.术后21 d,假手术组和对照组无结石生成;模型组每只大鼠膀胱内均有结石的生成,偏正光及傅里叶变换式红外光谱分析为磷酸铵镁与碳酸磷灰石混合成分的结石.结论 此感染性膀胱结石动物模型的设计是可行的,可以为进一步的实验提供帮助.
Objective To employ a newly modified rat model for infection-induced bladder stone formation.Methods 24 adult male Sprague Dawley rats were divided into 3 groups,model group （n =12）,sham operation group （n=8） and control group （n=4）.The surgical procedures were performed aseptically under anesthesia （25% Ultan 1.0 g/kg）.The bladder in model group was exposed through a short lower midline abdominal incision,the puncture needle（G18）with guideline was inserted aseptically into bladder,a metal wire ,which have been contaminated by the Proteus mirabilis ,was put into the puncture canal,then implanted into the bladder lumen through the guideline.In the sham operated group the puncture needle（Gl8） with guideline was inserted into bladder without metal wire implanted into the bladder.There was no any operation in control group.The rats were sacrificed by excessive anesthesia at 21 days post challenge.The bladder were removed aseptically and inspected for evidence of urolithiasis.Results On Day 2 after surgery,two rats died in model group,no rats died in other groups.Twenty-one days after surgery,all of rats in model group developed various-sized bladder stones.There was no stone formation in sham operation group and control group.All stones were verified by infrared spectroscopy and optical crystallography.These stone were struvite stone.Conclusion This model has a less trauma,faster recovery and excellent stone formation so that it may be used for the study of infection stone.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第34期2433-2435,共3页
National Medical Journal of China
关键词
膀胱结石
感染
模型
动物
Bladder calculi
Infection
Models,animal
