期刊文献+

Chondroitinase ABC treatment of injured spinal cord in rats Evaluation of long-term outcomes

Chondroitinase ABC treatment of injured spinal cord in rats Evaluation of long-term outcomes
下载PDF
导出
摘要 Chondroitin sulfate proteoglycans (CSPGs) which are produced by mature oligodendrocytes and reactive astrocytes can be upregulated after spinal cord injury and contribute to regenerative failure. Chondroitinase ABC (ChABC) digests glycosaminoglycan chains on CSPGs and can thereby overcome CSPG-mediated inhibition. However, many current studies have used an incomplete spinal cord injury model, and examined results after 8-12 weeks of ChABC treatment. In this study, a complete rat spinal cord transection injury model was used to study the long-term effects of ChABC treatment by subarachnoid catheter. Pathology of spinal cord regeneration was compared with control 24 weeks following ChABC treatment using immunohistochemistry and axon tracing techniques. At 24 weeks after injury, neurofilament 200 expression was significantly greater in the ChABC treatment group compared with the transection group. In the ChABC treatment group, axonal growth was demonstrated by a large number of biotinylated dextran amine positive axons caudal to, or past, the epicenter of injury. Biotinylated dextran amine-labeled fibers were found in the proximal end of the spinal cord in the transection alone group. These results confirm that ChABC can promote axon growth, neural regeneration, and repair after spinal cord injury in rats long after the initial injury. Chondroitin sulfate proteoglycans (CSPGs) which are produced by mature oligodendrocytes and reactive astrocytes can be upregulated after spinal cord injury and contribute to regenerative failure. Chondroitinase ABC (ChABC) digests glycosaminoglycan chains on CSPGs and can thereby overcome CSPG-mediated inhibition. However, many current studies have used an incomplete spinal cord injury model, and examined results after 8-12 weeks of ChABC treatment. In this study, a complete rat spinal cord transection injury model was used to study the long-term effects of ChABC treatment by subarachnoid catheter. Pathology of spinal cord regeneration was compared with control 24 weeks following ChABC treatment using immunohistochemistry and axon tracing techniques. At 24 weeks after injury, neurofilament 200 expression was significantly greater in the ChABC treatment group compared with the transection group. In the ChABC treatment group, axonal growth was demonstrated by a large number of biotinylated dextran amine positive axons caudal to, or past, the epicenter of injury. Biotinylated dextran amine-labeled fibers were found in the proximal end of the spinal cord in the transection alone group. These results confirm that ChABC can promote axon growth, neural regeneration, and repair after spinal cord injury in rats long after the initial injury.
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第16期1238-1242,共5页 中国神经再生研究(英文版)
基金 the National Natural Science Foundation of China,No.30471759
关键词 spinal cord injury chondroitinase ABC MORPHOLOGY TREATMENT biotinylated dextran amine anterograde tracing neural regeneration spinal cord injury chondroitinase ABC morphology treatment biotinylated dextran amine anterograde tracing neural regeneration
  • 相关文献

参考文献31

  • 1Basso DM,Beattie MS,Bresnahan JC.Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus ransaction.Exp Neurol.1996;139(2):244-256.
  • 2Nakamura M,Fujimura Y,Yato Y,et al.Changes in choline acetyltransferase activity and distribution following incomplete cervical spinal cord injury in the rat.Neuroscience.1996;75(2):481-494.
  • 3Nakamae T,Tanaka N,Nakanishi K,et al.Chondroitinase ABC promotes corticospinal axon growth in organotypic cocultures.Spinal Cord.2009;47(2):161-165.
  • 4Bradbury EJ,Moon LD,Popat RJ,et al.Chondroitinase ABC promotes functional recovery after spinal cord injury.Nature.2002; 416(6881):636-640.
  • 5Caggiano AO,Zimber MP,Ganguly A,et al.Chondroitinase ABCI improves locomotion and bladder function following contusion injury of the rat spinal cord.J Neurotrauma.2005;22(2):226-239.
  • 6De Wit J,De Winter F,Klooster J,et al.Semaphorin 3A displays a ransacti distribution on the surface of neuronal cells and interacts with proteoglycans in the extracellular matrix.Mol Cell Neurosci.2005;29(1):40-55.
  • 7Apostolova I,Irintchev A,Schachner M.Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice.J Neurosci.2006;26(30):7849-7859.
  • 8Dityatev A,Schachner M.The extracellular matrix and synapses.Cell Tissue Res.2006;326(2):647-654.
  • 9Iseda T,Okuda T,Kane-Goldsmith N,et al.Single,high-dose intraspinal injection of chondroitinase reduces glycosaminoglycans in injured spinal cord and promotes corticospinal axonal regrowth after hemisection but not contusion.J Neurotrauma.2008;25(4):334-349.
  • 10Barritt AW,Davies M,Marchand F,et al.Chondroitinase ABC promotes sprouting of intact and injured spinal systems after spinal cord injury.J Neurosci.2006;26(42):10856-10867.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部