摘要
背景:临床研究证实,糖尿病是引起牙周病变及牙槽骨丧失的危险因素,而糖尿病所致的高血糖对颌骨改变的影响机制目前仍未完全阐明。目的:观察高糖对体外培养的下颌骨成骨细胞增殖、分化、矿化的影响。方法:分离培养大鼠下颌骨成骨细胞,分别在生理糖浓度(5.5mmol/L;对照组)和高糖条件下(16.5mmol/L;高糖组)培养,采用MTT法、PNPP法、生化法、放射免疫法及茜素红S钙染法检测各组细胞的增殖能力、碱性磷酸酶活性、钙吸收、骨钙素分泌水平及矿化能力。结果与结论:高浓度的葡萄糖能显著促进成骨细胞的增殖(P<0.05),降低成骨细胞骨钙素的分泌(P<0.01),增加钙结节的数量及面积(P<0.01)。与对照组比较,高糖组成骨细胞碱性磷酸酶活性在21d时显著增高(P<0.01),钙吸收在14～21d时显著降低,而在21～28d时显著增高(P<0.01)。说明高糖能促进体外培养的大鼠下颌骨成骨细胞的增殖,延迟其分化和矿化。
BACKGROUND：Clinical research has demonstrated that diabetes mellitus is a risk factor for periodontal lesion and alveolar bone loss.However,it remains poorly understood the effect of hyperglycemia on changes of jaw bone.OBJECTIVE：To investigate the effects of hyglycemia on proliferation,differentiation and mineralization of osteoblasts from rat mandible.METHODS：Primary osteoblasts were isolated and incubated with medium containing 5.5 mmol/L and 16.5 mmol/L glucose,respectively.Cell proliferation,alkaline phosphatase（ALP） activity,calcium uptake,bone gla protein（BGP） and mineralization were detected using MTT,PNPP,biochemistry,radioimmunoassay,as well as Alizarin Red S staining.RESULTS AND CONCLUSION：High glucose could significantly increase cell proliferation（P 0.05）,decreased BGP level（P 0.01）,and increase number and the area of nodules（P 0.01）.Compared with the control group,the ALP activity of the high glucose group was obviously increased at 21 days after culture（P 0.01）;the calcium uptake was decreased at 14-21 days,but notably increased at 21-28 days（P 0.01）.These findings suggest that diabetes-associated hyperglycemia promotes rat mandibular osteoblasts proliferation while delaying differentiation and mineralization.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第33期6103-6107,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
