摘要
采用单因素试验和响应曲面试验优化灵芝药性固体发酵培养基。优化所得培养基组成为:以啤酒糟为基质,料水比1:1.4,黄芪12.52%,葡萄糖4.78%,KH2PO40.21%,MgSO4·7H2O0.25%,VB1微量。在此优化条件下,灵芪菌质多糖含量高达8.42mg/g,较优化前提高了29.54%。
Single-factor and response surface test were used for optimization of Ganoderma lucidum medicinal solid fermentation medium.Optimized medium composition were as follows：brewer＇s spent grain as substrate,the ratio of material to water was 1：1.4,Astragalus membranaceus 12.52%,glucose 4.78%,KH2PO40.21%,MgSO4·7H2O 0.25%,V B1 trace.Under the optimized conditions,GAPS concertration reached up 8.42mg/g,increased by 29.54% compared with unoptimization.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2010年第9期75-79,共5页
China Biotechnology
基金
"十一五"国家科技支撑计划重点项目(2008BADA1B05)资助项目
关键词
灵芝
黄芪
灵芪菌质多糖
固体发酵
啤酒糟
Ganoderma lucidum Astragalus membranaceus Ganodetma lucidum-Astragalus membranaceus polysaccharide（GAPS） Solid fermentation Brewer＇s spent grain
