摘要
目的为了正确认识和评估本实验室试验水平,更好地促进实验室试验能力建设,本实验室申请并参加了WHO的全球食源性感染网络(GFN)外部质量保证系统(EQAS)2009评估。方法分别用微量肉汤稀释法、玻片凝集法和16S rDNA鉴定法对EQAS邮寄的17株菌株盲样进行了药物敏感性测定、血清型分型鉴定和细菌种属鉴定。结果部分药敏结果有偏差(偏高或偏低);ESBLs(Extended Spetrumβ-Lactamase)检测及血清型鉴定结果完全正确;仅未知肠道细菌鉴定结果错误。结论以上事实表明,本实验室在某些细菌的复苏、鉴定,磺胺药物的药敏结果判读方面仍有不足,需加强和完善检测技术。
Objective In order to assess the testing level and promote the capability of our laboratory,we participated in an evaluation test conducted by the External Quality Assurance System of Global Foodborne Infections Network （GFN EQAS） in 2009.Method Seventeen strains of blind samples were tested on antimicrobial susceptibility,serotyping and strain identification by micro-dilution,slide agglutination and 16S rDNA identification,respectively.Results There were partial deviations on the results of drug susceptibility.The detection on ESBLs （Extended Spetrum β-Lactamase） and serotyping were absolutely correct but the identification of unknown enteric bacteria was wrong.Conclusion The facts indicated that the recovery and identification of bacteria,and the interpretation on the susceptibility of individual drugs needed to be improved.
出处
《中国食品卫生杂志》
北大核心
2010年第5期407-410,共4页
Chinese Journal of Food Hygiene
关键词
全球食源性感染网络
外部质量保证系统
血清分型
菌种鉴定
Global Foodborne Infections Network
External Quality Assurance System
Antimicrobial Susceptibility Test
Serotyping
Strain Identification
