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高迁移率族蛋白1对大鼠纤维母细胞样滑膜细胞增殖周期的调控作用 被引量：4

The regulatory effect of high mobility group box 1 on cell generation cycle of rat class fibroblast-like synovial cells
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摘要 目的:探讨高迁移率族蛋白(HMGB)1对滑膜细胞增殖周期的调控作用及可能机制。方法:将常规培养大鼠滑膜细胞株RSC364细胞随机分为正常对照组和10μg/LHMGB1刺激组,分别培养6、12和24小时,流式细胞术检测细胞周期分布和细胞中Cyclin D1/CDK4蛋白表达;免疫细胞化学检测PCNA和p21蛋白表达。结果:HMGB1作用至12和24小时,处于G0/G1期的细胞逐渐减少,G2/M期细胞增多,增殖指数升高分别为(68.00±1.42)和(69.61±5.86),与正常对照组(53.83±4.95)和6小时组(55.98±6.34)相比差异具有统计学意义(P<0.01或0.05)。PCNA和p21蛋白阳性信号均表达于滑膜细胞核内,随着作用时间延长,PCNA蛋白表达增强,阳性细胞数目逐渐增多,而p21蛋白表达减低,阳性细胞数目逐渐下降。HMGB1作用6～24小时CyclinD1蛋白相对表达量逐渐增加,分别为(1.42±0.02)、(1.65±0.03)和(1.67±0.01),与正常对照组(1.00±0.01)相比差异具有显著统计学意义(P<0.01),CDK4蛋白表达量也显著增加分别为(1.26±0.23)、(1.29±0.07)和(1.26±0.03),与正常对照组(1.00±0.0.25)相比差异具有显著统计学意义(P<0.01),但随着刺激时间的延长,其表达量无明显变化。结论:HMGB1可能通过上调细胞周期调控蛋白CyclinD1/CDK4的表达,下调细胞周期抑制剂p21的表达,促进滑膜细胞的增殖和分化。 Objective：To investigate the effect of high mobility group box （HMGB） 1 on cell generation cycle of RSC364 synoviocytes and possible mechanism.Methods：RSC364 cells induced by 10 μg/L HMGB1 were collected in 6 h,12 h and 24 h respectively,as well as normal control group cells in vitro.The cell cycle distribution state and the expression of Cyclin D1/CDK4 proteins were tested by flow cytometric analysis （FCM）,and then the expression of PCNA and p21 proteins was detected by immunocytochemistry（ICC）staining.Results：HMGB1 was significant effective on cell cycle distribution and proliferation index （PI） of RSC364 cells in 12 h and 24 h.The number of cells in G0/G1 phase was down-regulated,whereas cells in G2/M phase was up-regulated.Thus PI increased as well [（68.00±1.42） and （69.61±5.86） in 6 h,12 h respectively].Compared with those of the normal group （53.83±4.95） and 6 h HMGB1 induced group （55.98±6.34）,there were significantly statistical difference （P0.01 or 0.05）.ICC staining showed that the positive signal expression of PCNA and p21 was located in nucleus.The number of positive cells of PCNA increased and that of p21 decreased in time-dependently.HMGB1 could up-regulate the expression of Cyclin D1 proteins in time-dependent manner,they were （1.42±0.02）,（1.65±0.03） and （ 1.67±0.01） from 6 h to 24 h.Compared with that of normal group（1.00±0.01）,there were significantly statistical difference （P0.01）.HMGB1 could increased the expression of CDK4 protein also,they were （1.26±0.23）,（1.29±0.07） and （1.26±0.03） respectively.Compared with that of normal group（1.00±0.25）,there were significantly statistical difference either （P0.01）.No changes discovered among culture of 6 h,12 h and 24 h.Conclusion：HMGB1 could promote the proliferation of RSC364 cells by up-regulating the protein expression of Cyclin D1/CDK4,and by down-regulating p21 protein expression.
作者 郭惠芳 刘淑霞 周守勤 马丽艳 高丽霞 王志会
机构地区 河北医科大学第二附属医院免疫风湿科 河北医科大学病理教研室 河北省唐山钢铁集团公司职工医院检验科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2010年第9期832-835,共4页 Chinese Journal of Immunology
基金 河北省科技厅科技攻关基金(No.08206122D)资助项目
关键词 高迁移率族蛋白1 滑膜细胞 细胞周期 CYCLIN D1基因 P21 大鼠 High mobility group box chromosomal protein 1 Synoviocyte Cell cycle Cyclin D1 gene p21 Rat
分类号 R593.2 [医药卫生—内科学]
  • 相关文献

参考文献8

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二级参考文献12

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共引文献5

同被引文献28

  • 1孟明,顾立刚,张前,解华,王庆国.类风湿关节炎大鼠滑膜细胞的类肿瘤样增生与相关基因表达的关系[J].细胞与分子免疫学杂志,2005,21(4):411-414. 被引量：12
  • 2赵占胜,金玉怀,丛斌,李淑瑾,徐锦荣,姚玉霞,凌亦凌.八肽胆囊收缩素对TNF-α诱导的大鼠滑膜细胞株RSC-364 IL-6的作用及其可能的分子机制(英文)[J].中国病理生理杂志,2007,23(7):1396-1401. 被引量：8
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引证文献4

二级引证文献15

中国免疫学杂志
2010年 第9期

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