摘要
目的:探讨结肠腺癌及癌旁正常组织的microRNA分子表达谱的差异,为进一步研究相关microRNA在结肠腺癌发生、发展中的分子机制奠定基础。方法:选取3例新鲜的结肠腺癌组织及其癌旁正常粘膜组织,采用microRNA荧光探针杂交芯片扫描技术获得结肠腺癌和癌旁组织中的mi-croRNA表达谱,以样品间表达变化上下2倍作为阈值范围筛选差异表达的内源性microRNA。应用荧光实时定量聚合酶链式扩增技术(qRT-PCR)对表达谱的可靠性进行验证。结果:共获得差异表达的microRNA80个,其中上调30个,下调50个。分析得到变化具有统计学意义(P<0.05)的microRNA29个,其中上调2个,下调27个。qRT-PCR的结果与芯片结果一致。结论:结肠腺癌组织和正常组织存在差异表达的microR-NA分子,可能与结肠腺癌的发生、发展相关,microRNA有望成为结肠腺癌分子检测的重要指标及生物治疗的靶向基因。
AIM：To investigate the difference of microRNA （miRNA） expression profiles in colon adenocarcinoma and adjacent non-tumorous tissue （NT）,for further studies on molecular mechanism of miRNA on colon adenocarcinoma.METHODS：The fresh samples of 3 colon adenocarcinoma and NT were obtained and total RNA was isolated with TRIzol reagent.Hybridization was carried out on miRNA microarray chip.Quantitative Real Time polymerasechainreaction （qRT-PCR） was performed to confirm results obtained by microarray analysis.RESULTS：80 miRNAs with more than 2-fold change could be differentially expressed between NT and colon adenocarcinoma.27 human miRNAs were found significantly down-regulated,while 2 miRNAs was found significantly up-regulated （P〈0.05）.The expresson of miRNAs measured by qRT-PCR were consistent with that by miRNA microarray analysis in the same samples.CONCLUSION：There were differentially expressed miRNAs in colon adenocarcinoma and NT.These different miRNAs might be corrlated to the procress of carcinogenesis and progression of colon adenocarcinoma.The miRNAs expression profiles might be an important molecular biomarker in the diagnosis of colon adenocarcinoma and taget gene for tumor therapy.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第10期959-961,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
陕西省科技计划资助项目(2010K14-02)
