摘要
目的:观察淫羊藿苷(ICA)对环磷酰胺(Cy)所致小鼠骨髓和免疫抑制作用的影响,探讨ICA促进化疗后小鼠造血功能和免疫功能的作用及机制。方法:将小鼠随机分为6组:正常对照组,模型组,阳性对照组,ICA高、中、低剂量组。除正常对照组小鼠外,其余各组小鼠均腹腔注射Cy(200mg/kg)。第2天开始,对ICA高、中、低剂量的实验组小鼠灌胃不同剂量的ICA(150、80、40mg/kg.d),阳性对照组小鼠尾静脉注射参芪扶正注射液(1mL/d),模型对照组给予等量生理盐水,连续给予10d。经HE染色后观察小鼠胸腺组织结构的变化。用MTT比色法检测脾淋巴细胞的增殖率。用乳酸脱氢酶(LDH)法检测腹腔巨噬细胞对肿瘤细胞的杀伤能力。用ELISA试剂盒检测混合细胞培养上清中TNF-α和IL-12的含量。用全自动血液分析仪检测外周血红细胞、白细胞和血小板数量的变化;外周血和股骨骨髓涂片经瑞氏-吉姆萨染色后,光镜下计数单根股骨骨髓细胞(BMC)的数量。结果:ICA具有保护小鼠胸腺、骨髓免受Cy损伤的作用。不同剂量的ICA组小鼠的脾淋巴细胞增殖能力增加,巨噬细胞吞噬能力和分泌细胞因子的能力均增强,外周血红细胞、白细胞和血小板数量均明显上升。结论:ICA可逆转Cy化疗后小鼠骨髓造血和免疫功能的抑制状况。
AIM：To observe the effect of ICA on the immunosuppressive and bone-marrow-suppressive mice after chemotherapy,and explore the machanism of hematopoietic and immunologic function in mice accentuated by ICA.METHODS：Mice were injected Cy intraperitoneally except control group,then randomly divided into mode group（saline）,positive （Shenqi,1 mL/d）group,ICA groups（150,80,40 mg/kg·d）.All mice were treated respectively for 10 successive days.The pathological changes of thymus were observed by HE staining.Killing activity of peritoneal macrophage were measured by LDH kits and its production of TNF-α and IL-12 was measured by ELISA kits.Population of white blood cells （WBC）,red blood cell （RBC） and platelet （PLT） in the peripheral blood were detected with automated blood cell counter （ABCC）.Hemogram of peripheral blood and bone marrow morphology were observed under the microscope.RESULTS：ICA could protect the thymus and bone marrow from damage.The proliferation of lymphocyte and killing activity of macrophage cells in ICA treatment groups was all enhanced,moreover,the population of WBC,RBC and PLT were increased significantly.CONCLUSION：ICA can improve the state of immunosuppressive and myelosuppressive mice caused by Cy thus could alleviate the side effect of chemotherapy effectively.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第10期976-979,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
河北省中医药管理局课题(2009040)
