摘要
目的:观察哇巴因对白血病细胞株Jurkat、U937、K562、NB4、HL-60及Raji的增殖和凋亡的影响,并检测哇巴因对Jurkat细胞膜电位的影响。方法:四甲基偶氮唑盐(MTT)法检测哇巴因对不同白血病细胞株生长存活的影响,计算不同浓度哇巴因处理多种白血病细胞株的细胞增殖抑制率;瑞氏染色观察哇巴因对肿瘤细胞生长状态及形态的影响;AnnexinV/PI双染流式细胞仪检测哇巴因分别处理多种细胞的凋亡率;用DiBAC4(3)标记不同浓度哇巴因处理的Jurkat细胞膜电位,流式细胞仪检测细胞膜电位的变化。结果:MTT检测增殖结果表明哇巴因对Jurkat、K562、HL-60、NB4等细胞株均表现出显著增殖抑制作用;细胞形态学观察和凋亡检测结果表明50nmol/L哇巴因处理肿瘤细胞72h大量细胞表现出凋亡的形态特征,Jurkat细胞凋亡率显著增高;DiBAC4(3)标记Jurkat细胞膜电位发现哇巴因没有引起细胞膜的去极化。结论:哇巴因对多种白血病细胞株具有很强的增殖抑制和凋亡诱导作用;哇巴因的增殖抑制和凋亡诱导作用并没有引起Jurkat细胞膜电位的改变,其作用的浓度未引起钠钾ATP酶的抑制。
Objective：To observe the effects of ouabain on the proliferation and apoptosis in different leukemia cell lines Jurkat,U937,K562,NB4,HL-60,Raji,and probe the effects of different dosages of ouabain on jurkat cell membrane potential.Methods：Using the MTT method for assaying the inhibition degree of cell proliferation.The morphological changes in leukemia cell lines were observed after wright staining.Cell apoptosis was determined by flow cytometry after Annexin V /PI staining.Jurkat cell membrane potential was labeled by DiBAC4（3）and measured by flow cytometry.Results：MTT assay showed that ouabain could significantly inhibit the proliferation of leukemia cell lines.Wright＇s staining and apoptosis detection indicated dramatic proapoptotic morphological changes and enhanced cell apoptosis rate in Jurkat,K562,HL-60 and NB4 cell lines after treatment with ouabain.Cell membrane potential detection showed ouabain didn＇t induce the depolarization of Jurkat cell membrane.Conclusion：Ouabain has potent antileukemia activity and can significantly inhibit the proliferation and induce apoptosis in leukemia cell lines,meanswhile there were no inhibition effect on the Na,K-ATPase of ouabain.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第9期1245-1248,1378,共5页
Journal of Nanjing Medical University(Natural Sciences)
关键词
哇巴因
白血病
增殖
凋亡
leukemia
ouabain
proliferation
apoptosis
