摘要
目的:探讨受精液中趋化因子5(CCL5)与卵母细胞发育潜能的关系。方法:收集25个体外受精周期中获得的180枚卵母细胞的受精液,按卵母细胞的发育命运分为4组:A组为35枚未受精卵母细胞的受精液;B组为72枚发育为非优质胚胎卵母细胞的受精液;C组为70枚发育为优质胚胎卵母细胞的受精液;D组为11枚发育为妊娠胚胎卵母细胞的受精液。ELISA方法测定4组中CCL5的含量,并将结果进行比较。结果:A组CCL5的含量最低,与其余3组比较差异均有统计学意义(P<0.05)。C组与B组比较差异有统计学意义(P<0.05),但与D组比较差异无统计学意义(P>0.05)。结论:卵丘细胞分泌的CCL5水平与卵母细胞的受精及发育潜能相关,可作为选择移植胚胎的新的参考标准。
Objective：To study the correlation between the level of CC chemokine CCL5 in fertilization fluid and oocytes developmental potential.Methods：The fertilization fluid of 180 oocytes received from 25 IVF cycles were divided into four groups according to oocytes developmental destiny：group A： fertilization fluid of 35 unfertilized oocytes; group B： fertilization fluid of 72 oocytes destined for poor-quality embryos; group C： fertilization fluid of 70 oocytes destined for good-quality embryos; group D： fertilization fluid of 11 oocytes destined for pregnant embryos. ELISA was used to observe and analyze the expression of CCL5 in the four groups.Results：Compared with group B,C,D, the level of CCL5 in group A was significantly lowest; Group C was significantly different from group B, but not different from group D.Conclusion：There is correlation between CCL5 secreted by cumulus cells and oocytes fertilization and developmental potential. The level of CCL5 may offer scientific proof in selecting implanted embryos.
出处
《中国妇幼保健》
CAS
北大核心
2010年第27期3941-3943,共3页
Maternal and Child Health Care of China
关键词
卵丘细胞
趋化因子5
卵母细胞
发育潜能
Cumulus cells
CC chemokine CCL5
Oocytes
Developmental potential
