摘要
建立了谷氨酸棒杆菌S9114合成L-谷氨酸(L-Glu)的代谢流平衡模型,应用该模型并结合S9114在10%和30%溶氧条件下发酵至中后期时胞外相关产物的分泌情况计算出其代谢流分布;通过MATLAB线性规划得到了谷氨酸合成的理想代谢流分布。代谢流分析结果表明,减少HMP和TCA循环的代谢流量,增加CO2固定的代谢流将有利于谷氨酸的合成;在谷氨酸发酵工艺控制中,溶氧是关键因素,发酵中后期提高溶氧有利于谷氨酸的生成,同时可抑制副产氨基酸的产生;主要副产物乳酸在理想代谢流分布中仍占有一定比例,说明乳酸在整个代谢过程中起到一定的作用。
The metabolic flux balance model of biosynthesis of L-glutamic acid by Corynebacterium glutamicum S9114 was constructed. Metabolic flux distribution was calculated with combination of the model and situation of extracellular metabolites of S9114 in fermentation phase of medium to later under capacity of dissolved oxygen by 10% and 30%. And ideal glutamic acid metabolic flux distribution was obtained with linear program of MATLAB software. The results of metabolic flux analysis indicated that synthesis of glutamic acid will be increased when increase metabolic flux of CO2 anaplcrotic reactions and decrease metabolic flux of TCA cycle and HMP pathway. Dissolve of oxygen was the key factor of fermentation control, and increase of rate of dissolved oxygen in medium in later phase of fermentation would improve glutamate biosynthesis and inhibit production of by-product amino acids. Main by-product lactic acid occupied certain ratio in ideal glutamic acid metabolic flux distribution, which indicated that lactic acid might play a role in the process of glutamate biosynthesis.
出处
《中国酿造》
CAS
北大核心
2010年第10期72-76,共5页
China Brewing
基金
国家"十一五"科技支撑计划(2007BAK36B03)
关键词
谷氨酸棒杆菌
谷氨酸
代谢流分析
溶氧
Corynebacterium glutamicum
L-glutamic acid
metabolic flux analysis
dissolved oxygen
