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基于SAS的杀虫剂毒力筛选计算机实现 被引量:5

SAS-based computer screening of insecticidal toxicity
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摘要 目的采用国际通用的SAS统计分析平台编制计算机程序,实现杀虫剂毒力的快速筛选。方法以德国小蠊为试虫,对A、B2种杀虫剂进行室内熏蒸毒力测定,对所得数据进行计算机程序演算,以加权概率单位法拟合毒力回归线,计算2种药剂的LC50及其95%置信限(95%CI),并对2种药剂LC50及毒力回归线斜率进行两两分析比较。结果 A、B药剂杀灭德国小蠊的毒力回归线分别为Y(probit)=-3.193+5.005×Log1(0dose)和Y(probit)=-2.498+2.696×Log1(0dose);参数效应Waldχ2检验的P值均<0.01,方程拟合优度检验的P值均>0.05。2种药剂的LC50值分别为4.35g/m3(95%CI:3.17~5.29g/m3)和8.44g/m3(95%CI:6.07~10.66g/m3);毒力线斜率分别为5.005和2.696。A、B药剂LC50两两比较结果:u=3.415,P<0.001;斜率间两两比较结果:t=2.028,P<0.05。提示从药剂的毒力和虫群敏感性而言,A药剂均强于B药剂。结论建立的快速筛选方法及编制的程序计算结果经双重验证,证明准确、可靠,且简便、快捷。 Objective A computer program was formulated on the internationally recognized platform of statistics analysis system(SAS)for rapid screening of pesticide toxicity.Methods Two insecticides,A and B,were tested for the toxicity against Blattella germanica using indoor fumigation.The resulting data were subject to computation by this program to fit a weighted probit regression line.The LC50 and 95% confidence interval(95%CI)of both agents were then calculated and compared in pair with the slope of the toxicity regression line.Results The toxicity regression lines of the two agents were Y(probit)=-3.193+5.005×Log10(dose)and Y(probit)=-2.498+2.696×Log10(dose).The Wald test showed both P values were 0.01,while the goodness-of-fit test showed both P values were〉 0.05.The LC50 values for the two pesticides were 4.35 g/m^3(95%CI:3.17-5.29 g/m^3)and 8.44 g/m^3(95%CI:6.07-10.66 g/m^3),respectively; the slopes of the two regression lines were 5.005 and 2.696,respectively.Comparison between the LC50 values showed:u=3.415,P〈0.001; and that between slopes revealed:t=2.028,P 0.05.These results suggested that agent A was stronger than B in both toxicity and sensitivity.Conclusion Through dual verification,the established method and computer program proved to be accurate,reliable,and simple and fast for rapid screening of insecticidal toxicity.
出处 《中国媒介生物学及控制杂志》 CAS CSCD 2010年第5期478-481,共4页 Chinese Journal of Vector Biology and Control
基金 国家质检总局科研项目(2003IK062)~~
关键词 毒力测定 筛选 SAS 程序 熏蒸 Toxicity measurement Screening Statistics analysis system Program Fumigation
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