摘要
目的探讨甘糖酯对脑缺血-再灌注损伤大鼠海马谷氨酸转运体功能的影响。②方法采用大鼠三血管夹闭、松夹制作脑缺血-再灌注损伤模型,利用海马突触膜颗粒对3H-L-谷氨酸摄入量的测定,观察了甘糖酯对谷氨酸转运体功能的影响,同时测定了脑组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的变化。③结果缺血-再灌注损伤时,大鼠海马谷氨酸转运体功能及SOD活性明显降低,MDA明显升高,与对照组比较差异均有显著性(t=3.691~5.406,P均<0.01);应用甘糖酯组大鼠海马谷氨酸转运体功能及SOD活性显著提高,MDA含量降低,与缺血-再灌注损伤组相比,差异均有显著性(t=2.938~3.127,P均<0.05)。④结论甘糖酯可能通过清除自由基的作用,改善大鼠脑组织缺血-再灌注损伤时海马谷氨酸转运体的功能。
Objective To investigate the effect of proplylene glucol mannurate sulphate(
PGMS) on glutamate transporter function of hippocampus in ischemiareperfusion injured
(I120R30) rats. Methods The brain ischemiareperfusion model of rat was made by clipping and
releasing three arteries.Glutamate transporter function was studied by assay of 3HLglutamate
uptake in hippocampus synaptosomes. MDA contents and SOD activities were detected by
spectrophotometry. Results The glutamate transporter function and SOD activities of
hippocampus in ischemiareperfusion rats were significantly decreased(t=4.482,3.691,P<0.01),
contents of MDA were increased(t=3.067,P<0.05) compared with control group. Glutamate
transporter function and SOD activity in PGMS group were increased(t=3.127,2.938,P<0.05),
contents of MDA were decreased(t=3.067,P<0.05) compared with ischemiareperfusion rats.
Conclusion PGMS can improve glutamate transporter function of ischemiareperfusion rats
through the elimination of free radicals.
出处
《青岛医学院学报》
1999年第1期32-33,共2页
Acta Academiae Medicinae Qingdao Universitatis
基金
国家自然科学基金
关键词
甘糖酯
谷氨酸转运体
再灌注损伤
大鼠
脑缺血
proplylene glucol mannurate sulphate
glutamate transporter
brain ischemiareperfusion injury
lipid peroxidation
rat