摘要
目的:建立RP-HPLC同时测定黄连中药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀及小檗碱等6种生物碱含量的方法。方法:XtimateTM C18色谱柱(4.6mm×250mm,5μm);流动相为乙腈-30mmol·L-1碳酸氢铵水溶液(含有0.7%氨水、0.1%三乙胺),线性梯度洗脱;检测波长270nm;柱温30℃;流速1.0mL·min-1。结果:6种生物碱分别在0.85～16.96(r=0.9997),1.25～24.96(r=0.9995),2.05～40.96(r=0.9999),3.65～72.96(r=0.9999),2.88～57.60(r=0.9998),13.25～264.96mg·L-1(r=0.9996)具有良好的线性;平均加样回收率(n=6)依次为101.6%(RSD1.3%),102.5%(RSD1.5%),100.8%(RSD1.9%),102.6%(RSD1.2%),97.80%(RSD1.3%),99.01%(RSD1.5%)。不同产地黄连中6种被测生物碱及总生物碱存在显著的差异。结论:该方法准确、可靠,重复性好,可用于同时测定黄连中药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀及小檗碱的含量。
A reversed-phase HPLC method for simultaneous determination of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in Coptis chinensis was developed. Analysis was carried out on an XtimateTM C18 column （4.6 mm×250 mm, 5 μm）eluted with acetonitrile-30 mmol·L-1 ammonium bicarbonate solution（including 0.7% ammonia and 0.1% triethylamine） by gradient elution. The detective wavelength was 270 nm, the column temperature was 30 ℃, and the flow rate was 1.0 mL·min-1. By the above method, the linear ranges of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine were 0.85-16.96 （r=0.999 7）, 1.25-24.96 （r=0.999 5）, 2.05-40.96 （r=0.999 9）,3.65-72.96 （r=0.999 9）,2.88-57.60 （r=0.999 8）,13.25-264.96 mg·L-1（r=0.999 6）, respectively. The average recoveries （n=6） of the six alkaloids were 101.6%（RSD 1.3%）,102.5%（RSD 1.5%）,100.8%（RSD 1.9%）,102.6%（RSD 1.2%）,97.80%（RSD 1.3%）,99.01%（RSD 1.5%）, respectively. The determined results demonstrate that there is a significant difference in the contents of six alkaloids and total alkaloids among the tested samples. The method is accurate, reliable and repeatable for simultaneous determination of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in C. chinensis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第19期2576-2580,共5页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划项目(2007BAI40B05
2007BAI40B03)
