摘要
目的研究心脏骤停缺血/再灌注损伤前后血浆内皮素、降钙素基因相关肽、丙二醛和血Na+、K+、Cl-、Ca2+的含量变化规律及意义。方法实验组采用主动脉与腔静脉钳夹法建立心脏骤停缺血/再灌注损伤模型,对照组动物麻醉、气管插管、开胸暴露心脏、主动脉与腔静脉过程同实验组,但不行主动脉和腔静脉钳夹。结果实验组心脏骤停时表现为电机械分离,平均动脉压(MAP)2.44±2.14 kPa。复苏后30 min血浆ET显著升高(P<0.01),复苏后2 h仍维持在较高水平(P<0.05);而血浆CGRP含量在复苏后5 min尚处于较低水平(P<0.05),复苏后2 h显著升高(P<0.05);血MDA、K+均于复苏后30 min显著升高(P<0.01)。实验组血Na+、Cl-、Ca2+和对照组的各项指标复苏前后均无显著性变化(P>0.05)。结论ET、CGRP变化及相关关系可能是心脏骤停缺血/再灌注损伤过程中一种调节机制并在病理生理过程中起重要作用,同时心脏骤停复苏过程中,有效调节和改善内环境也是心肺复苏成功的重要因素。
Objective To study the changes of ET,CGRT、MDA and Na＋,K＋,Cl-,Ca＋＋ in blood of rabbit with resuscitation of cardiac arrest. Methods The experimental animals with ischemia and reperfusion injury by cardiac arrest were bound by clamping the aortic and vena cava.The anesthesia method,tracheal intubation and the exposure of heart,aortic,vena cava of control animals were as the same as that of experimental animals,but their aortic and vena cova were not clamped. Results The experimental animals expressed electrical mechanical separation with cardiac arrest,the mean artery pressure（MAP） was 2.44±2.14 kPa.ET in blood highly increased after resuscitating 30 mins（P0.01）.It also remained higher level after resuscitating 2 hours（P0.05）.Serum CGRP remained lower level after resuscitating 5 mins,but it increased significantly after resuscitating 2 hours（P0.05）.Serum MDA,K＋ significantly increased after resuscitating 30 mins（P0.01）.Serum Na＋,Cl-,Ca＋＋ in experimental animals and all indexes of control animals had no significantly changes.Conclusion The changes of serum ET,CGRP and their correlation may play an important role in resuscitation of cardiac arrest.
出处
《河南职工医学院学报》
2010年第5期523-525,共3页
Journal of Henan Medical College For Staff and Workers
