摘要
目的:超声波法优选苦豆子中总黄酮的提取工艺。方法:以超声波为提取方法,以总黄酮提取率为考察指标,选取乙醇浓度(体积分数)、加溶剂倍量和超声时间为考察因素,采用均匀实验和正交实验,优选苦豆子中总黄酮的提取工艺。结果:影响总黄酮提取率各因素主次为:A>C>B,确定最佳提取工艺为A2B3C3,即70%乙醇,加溶剂40倍量,超声提取75 min。结论:采用该工艺提取苦豆子中总黄酮,提取率较高且稳定,说明该工艺合理可行。
Objective： To optimize the extraction process of total flavonoids from Sophora alopecuraides.Methods： The extracting method is ultrasonic,extraction ratio of the total flavonoids as the target,selecting the ethanol concentration（volume fraction）,solid-liquid ratio and the ultrasonic time as study factors,using uniform test and orthogonal experiment,optimizing the extraction process of total flavonoids from Sophora alopecuroides.Results： The order of various factors on the extraction rate is ACB,the determined extraction process is A2B3C3,namely the concention of the ethanol is 70%,the solid-liquid ratio is 40（g： mL）,the ultrasonic time is 75 min.Conclusion： Experiment results make it clear that because the rate of total flavonoids extracted from Sophora alopecuroides by the optimum extracting process is high and stable,the process is reasonably practicable.
出处
《中国野生植物资源》
2010年第5期35-38,共4页
Chinese Wild Plant Resources
基金
宁夏回族自治区自然基金项目(NZ0859)
关键词
苦豆子
总黄酮
均匀实验
正交实验
Sophora alopecuroides
total flavonoids
uniform experiment
orthogonal experiment
