摘要
[Objective]The research aimed to study the regeneration technology of Cazania rigens L.leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L.leaves.[Method]Using Japan imported C.rigens leaves as materials,the orthogonal test was made for the callus and adventitious buds induction in MS medium with different kinds and concentrations of hormones.The optimum medium formula for the regeneration of C.rigens leaves were screened out.[Result]On the medium of MS ＋ 0.8-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,compact type and bright green calli were formed.When the leaves were inoculated on the medium of MS ＋ 0.5-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,many adventitious shoots can be induced and the induction rate reached 100%.When strong adventitious shoots with the height of 2.0-3.0 cm were transplanted into the medium of 1/2 MS ＋0.1 mg/L NAA,the rooting situations were good and the rooting rate was 100%.[Conclusion]The research provided a new way for the rapid propagation of C.rigens and laid the foundation for the genetic transformation and new varieties breeding of C.rigens.
[目的]研究勋章菊叶片再生技术,筛选适合勋章菊叶片再生的最佳培养配方。[方法]以日本引进的勋章菊叶片为材料,置床于添加不同种类和浓度激素的MS培养基中进行愈伤组织和不定芽诱导的正交试验,筛选勋章菊叶片再生的最佳培养基配方。[结果]在MS+TDZ(0.8～1.0mg/L)+NAA(0.05～1.0mg/L)培养基上可形成紧密型绿色的愈伤组织;叶片接种于MS+TDZ(0.5～1.0mg/L)+NAA(0.05～1.0mg/L)培养基上可形成许多不定芽,诱导效率达100%;健壮的不定芽长至2.0～3.0cm长时移至1/2MS+NAA(0.1mg/L)培养基中,其发根状况良好,生根率达100%。[结论]为勋章菊的高频快繁提供了新途径,并为勋章菊的遗传转化及培育新品种奠定基础。
基金
Supported by the Extra-curricular Academic Research Found of 12th Batch of Students in Soochow University (KY2010114A)
Science and Technology Support (Agriculture) Project of Suzhou Province(SNG0908)~~
