摘要
为了研究高密度脂蛋白对抗动脉粥样硬化的作用机理，采用酶联免疫受体分析法对注射高密度脂蛋白的动脉粥样硬化家兔肝细胞膜高密度脂蛋白受体活性进行了检测。结果表明，人血浆高密度脂蛋白虽不能降低高脂饲养的家兔血脂含量，但可显著降低其肝脏脂质水平和显著升高胆囊胆汁脂质水平。受体分析发现，高脂对照组动物肝细胞膜高密度脂蛋白受体Ｋｄ值显著降低，而高密度脂蛋白试验组动物Ｋｄ值未见明显变化，但Ｂｍａｘ却显著降低。提示长期高脂饲养有使动脉粥样硬化模型动物肝细胞膜高密度脂蛋白受体Ｋｄ值显著降低的作用。
Aim In an effect to study further the anti-atherogenic effects of human plasma HDL, the HDL receptor activity on liver plasma membranes of cholesterol fed As rabbits injected with HDL was investigated. Methods HDL receptor activity was detected by the method of enzyme linked immunosorbent receptor assay (ELISA) and the levels of serum, liver and bile lipids of As rabbits were also determined. Results The results of lipid measurement showed that human plasma HDL could not decrease the level of serum lipids of cholesterol fed rabbits, but decreased significantly the levels of live lipids and increased significantly the levels of bile lipids of As rabbits compared with As rabbits injected with saline. The results of HDL receptor assay showed that the value of Kd was significantly lower in As rabbits than that in normal rabbits (P<0.01). In HDL treated rabbits, no difference was observed with Kd(P>0.05) while Bmax reduced significantly(P<0.05) compared with normal rabbits. Conclusion These results suggested that Kd of rabbit liver plasma membrane HDL receptor reduced significantly when the rabbits were fed with cholesterol rich diet for a long period(22 weeks), whereas HDL seemed to ameliorate or avoid this change.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期125-127,共3页
Chinese Journal of Arteriosclerosis
基金
国家新药研究基金
关键词
动脉粥样硬化
高密度脂蛋白
肝
细胞膜
受体
Atherosclerosis
Rabbits
Lipoprotein, HDL
Receptors
Liver
Cell Membrane
Activity
Lipids
