摘要
为研究氧化型低密度脂蛋白和维生素Ｅ对人脐静脉内皮细胞产生一氧化氮及合酶的影响，应用一氧化氮及一氧化氮合酶试剂盒测定培养的人脐静脉内皮细胞一氧化氮含量及一氧化氮合酶活性。结果发现，氧化型低密度脂蛋白能显著抑制培养的内皮细胞一氧化氮的产生，从对照组的１２６±２４ｍｍｏｌ／ｇ下降为４１±８ｍｍｏｌ／ｇ；并能明显降低一氧化氮合酶的活性，具有浓度和时间效应。抗氧化剂维生素Ｅ显著增加氧化型低密度脂蛋白刺激内皮细胞产生一氧化氮含量以及一氧化氮合酶活性。提示氧化型低密度脂蛋白可以通过抑制内皮细胞一氧化氮合酶的活性而抑制其产生一氧化氮。维生素Ｅ通过增加一氧化氮合酶活性而增加内皮细胞产生一氧化氮。
Aim To study the effect of oxidized low density lipoprotein(ox LDL) and antioxidant vitamin E (Vit E)on nitric oxide (NO) production and nitric oxide synthase(NOS) in cultured human umbilical vein endothelial cells (hUVEC). Methods The level of NO and NOS activity were measured by NO and NOS kits in hUVEC. Results ox LDL significantly decrease NO production from 126±24 mmol/g to 41±8 mmol/g and the activity of NOS in a dose and time dependent manners in hUVEC. Vit E can markedly increased ox LDL induced NO production and the activity of NOS in hUVEC. Conclusion ox LDL can markedly attenuate the NO synthesis, the possible mechanism of this effect was attributed to the decrease of NOS activity. Vita min E can elevated the production of NO via increasing the NOS activity.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期145-148,共4页
Chinese Journal of Arteriosclerosis
关键词
低密度脂蛋白
抗氧化剂
内皮细胞
维生素E
NO
Lipoprotein
LDL
Oxidants
Antioxidants
Umbilical Veins
Endothelial Cells
Nitric Oxide
Nitric Oxide Synthase
Vitamin E
