摘要
用荧光光谱、紫外吸收光谱及圆二色光谱技术研究Fe3O4磁性纳米粒子与透明质酸酶(Hyaluronidase)的相互作用,结果表明Fe3O4磁性纳米粒子对透明质酸酶的荧光猝灭机理为静态猝灭;二者结合常数K在104数量级,说明Fe3O4磁性纳米粒子与透明质酸酶有较强的结合;二者相互作用过程中各热力学常数的变化表明Fe3O4与透明质酸酶的作用过程是一个自发过程,而且他们之间的结合力为疏水作用力;紫外吸收光谱研究发现Fe3O4能改变透明质酸酶的空间结构;圆二色谱数据表明Fe3O4磁性纳米粒子能够引起透明质酸酶的二级结构发生变化。
The interaction of Fe3O4 magnetic nanoparticles with Hyaluronidase was investigated by fluorescence,ultraviolet and circular dichroism spectral method （CD）. The experimental results demonstrated that the quenching mechanism of Fe304 magnetic nanoparticles to Hyaluronidase fluorescence was a static quenching due to existence of non-radiative energy-transfer. The binding constant of Fe3O4 magnetic nanoparticles with Hyaluronidase is four factors of ten,which indicate there is strong association between them. The changes of the thermodynamic parameters showed that the main interaction forces are electrostatic reaction and the hydrophobic interaction between Fe304 magnetic nanoparticles and Hyaluronidase. At the same time,through the ultraviolet absorption and circular dichroism（CD） spectroscopy,it showed that the secondary structure of Hyaluronidase is changed induced by the binding of Fe3O4 magnetic nanoparticles.
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2010年第3期51-55,共5页
Journal of Guangxi Normal University:Natural Science Edition
基金
广西自然科学基金资助项目(2010GXNSFF013001)
广西应用基础研究专项资助项目(0639028)
广西教育厅研究生科研创新项目
广西师范大学青年骨干教师基金资助项目
关键词
Fe3O4磁性纳米粒子
透明质酸酶
荧光光谱
紫外光谱
圆二色谱
Fe3O4 magnetic nanoparticles
hyaluronidase
fluorescence spectroscopy, ultraviolet
circular dichroism （CD）
