摘要
目的：直接进样高效液相色谱法测定人血浆中的头孢唑林、头孢曲松、头孢唑肟的含量。方法：选用浸透限制固定相，紫外检测器，使三种头孢菌素分别在流动相ｐＨ５．７５、５．２５和４．３５条件下与内标及血浆中的蛋白质得到良好的分离。结果：制作了三种头孢菌素各自的标准曲线，测定方法的变异系数在１．２％～７．１％，回收率近１００％。实测了６例临床静脉输注头孢唑林、头孢曲松、头孢唑肟病人的血药浓度。结论：浸透限制固定相色谱法测定血浆中的头孢菌素，省去了前处理，快速简单，更适用于临床治疗药物监测。
Purpose: To establish a new method to determine cephalosporines in plasma by high
performance liquid chromatography (HPLC). Metheds: Cefazoline,ceftriaxone and ceftizoxime
in plasma were determined by HPLC with restricted access stationary phases and UV
detector.Their standard curves were calculated.The concentrations of them in plasma of 6
patients after iv were analysed. Results: The 3 cephalosporines were well separated from the
internal standard and proteins in pH 5.75,5.25 and 4.35 respectively. The recovery rates were
98.2%100%,and the coefficients of variation were 1.2%7.1%. Conclusion:5BZThis new method
established to determine cephalosporines in human plasma by HPLC with restricted access
stationary phases is simple and rapid,and it may be applicable to routine use in clinic.
出处
《西北国防医学杂志》
CAS
1999年第2期86-88,共3页
Medical Journal of National Defending Forces in Northwest China
基金
全军95医学科研基金
关键词
头孢菌素
浸透限制固定相
HPLC
直接进样
Cephalosporines
Restricted access stationary phases
High performance liquid
chromatography
