摘要
目的:建立同时测定大黄中大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素、番泻苷A和番泻苷B含量的方法。方法:色谱柱为Agilent Zorbax SB-C18(150mm×4.6mm,5μm),流动相为0.05%磷酸水溶液-乙腈,梯度洗脱,流速为1mL.min-1,检测波长254nm。结果:7条标准曲线在检测范围内均呈良好线性(r≥0.9999);7个待测成分的检测限均低于0.18ng,定量限均低于0.48ng;高、中、低3个水平日内和日间精密度的RSD均小于3.1%,加样回收率为92.0%～104.1%。应用所建立的方法测定了10个采自不同地区不同种的大黄样品中以上7个成分的含量,其结果差别较大。结论:本文所建立的大黄中7个指标成分同时定量的方法准确、可靠,可作为大黄药材质量控制的参考方法。
Objective：To establish the method for simultaneous determination of 7 anthraquinione derivatives from rhubarb,including emodin,rhein,chrysophanol,physcion,aloe-emodin,sennoside A,and sennoside B.Methods：The analysis was achieved with an Agilent Zorbax SB-C18 analytical column （150 mm×4.6 mm,5 μm）by gradient elution of 0.05%（v/v）phosphoric acid in water and acetonitrile.The flow rate was 1 mL·min-1,and detection wavelength was set at 254 nm.Results：All the calibration curves showed good linearity（r≥0.9999）in a relatively wide concentration range.The LOD and LOQ were lower than 0.18 ng and 0.48 ng on column,respectively.The RSD for intra-and inter-day of 7 analytes was less than 3.1% at three levels,and the recoveries were 92.0%-104.1%.The validated method was successfully applied for the analysis of 7 anthraquinone derivatives in different species of rhubarb which had great variation on the content of investigated anthraquinones.Conclusion：The developed HPLC assay is accurate and reliable,and can be readily utilized as a quality control method for rhubarb.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第9期1636-1641,共6页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱
同时测定
大黄
蒽醌类化合物
HPLC
simultaneous determination
rhubarb
anthraquinone derivatives
