摘要
目的:建立高效液相色谱法同时测定六味安消胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5个成分含量。方法:采用DikmaC18(200mm×4.6mm,5μm)色谱柱,流动相为甲醇(A)-0.1%磷酸溶液(B)进行梯度洗脱,流速1mL.min-1,检测波长254nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进样量分别在22.20～599.4,35.14～948.8,33.79～912.3,49.2～1328.4,15.72～424.4ng范围内线性关系良好。平均回收率(n=6)分别为101.9%,98.8%,102.5%,102.9%,99.7%;RSD分别为1.4%,2.0%,1.5%,0.83%,2.4%。结论:该法分析简便、准确,可作为该制剂的定量分析方法。
Objective：To develop an HPLC method for determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Liuwei Anxiao capsules.Methods：Dikma C18（200 mm×4.6 mm,5 μm） column was adopted.The mobile phase was composed of methnol（A） and 0.1% phosphoric acid（B） with gradient elution.The flow rate was 1 mL·min-1 and the detection wavelength was 254 nm.Results：The linear ranges of aloe-emodin,rhein,emodin,chrysophanol and physcion were 22.20-599.4,35.14-948.8,33.79-912.3,49.2-1328.4,and 15.72-424.4 ng,respectively;The average recoveries were 101.9%（RSD=1.4%）,98.8%（RSD=2.0%）,102.5%（RSD=1.5%）,102.9%（RSD=0.83%）,99.7%（RSD=2.4%）,respectively.Conclusion：The method is convenient,accurate and can be used for the determination of the preparation.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第10期1841-1844,共4页
Chinese Journal of Pharmaceutical Analysis
