摘要
目的制备杨梅素微乳,并评价其质量。方法选择适宜的油相、表面活性剂和助表面活性剂,利用伪三元相图筛选微乳处方,研制出适合经口给药的微乳制剂;采用HPLC法测定杨梅素的含量,并对微乳的类型、载药量、体外释放等进行考察。结果优选处方为聚山梨酯80-聚山梨酯20-乙醇-油酸-pH 6.5磷酸缓冲液的质量比20∶10∶15∶5∶50,杨梅素质量分数为2%。所制微乳外观透明,平均粒径为(56.3±8.7)nm,zeta电位为-2.73 mV,电导率为13.27 mS.m-1。载药微乳在不同pH值环境下对药物有良好的释放效果。结论通过优化处方制备的杨梅素微乳具有较稳定的理化性质,HPLC法可以有效、准确的测定杨梅素微乳的含量,并且微乳载药系统有良好的释药效果。
Objective To prepare myricetin microemulsion and estimate its quality. Methods The formulation was investigated by pseudo-ternary phase diagrams obtained by titration method. Myricetin was determined by HPLC. The type of the microemulsion, in vitro release and carrying dosage were investigated. Results The optimized formulation was composed of Tween 80-Tween 20-ethanol-oleic acid-pH 6.5 phosphate buffer （ m： m： m ： m ： m = 20 ： 10 ： 15 ： 5 ： 50 ） and the drug loading amount was 2%. The average diameter was （56. 3 e 8. 7） nm; the zeta potential was - 2. 73 mV; the electrical conductivity was 13. 27 mS.m^- 1. Dosages in microemulsion were slow-released in 24 h at different pH conditions. Conclusions By optimized microemulsion formulation, the prepared myricetin microemulsion possess stable physical and chemical properties, the content of myricetin microemulsion can be determined by HPLC method, and microemulsion system shows a good drug release effect.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2010年第10期777-783,共7页
Journal of Shenyang Pharmaceutical University
关键词
杨梅素
微乳
伪三元相图
高效液相色谱法
体外释放
myricetin
microemulsion
pseudo-ternary phase diagram
HPLC
releasing in vitro
