摘要
目的:研究在氟中毒引起氟斑牙时,氟对大鼠切牙成釉细胞DNA损伤的影响。方法:给雄性SD大鼠饮用含10、50、100mg/LNaF的高氟水120d,制备氟斑牙模型,用单细胞凝胶电泳检测DNA的损伤。结果:雄性SD大鼠饮用高氟水后,血清氟含量较对照组显著增高,P<0.05。饮水氟含量与血清氟含量的相关系数为0.9153(P<0.05),具有较好的剂量-效应关系,大鼠切牙呈现典型的氟斑牙改变。在50mg/LNaF的剂量条件下,大鼠切牙成釉细胞彗星长与对照组比较,P<0.05。在100mg/LNaF的剂量条件下,彗星长、Olive尾距、尾分布距与对照组比较,P<0.05,而尾长值虽比对照组增加,但P>0.05。低剂量染氟组(10mg/LNaF)大鼠切牙成釉细胞DNA损伤情况与对照组比较,没有显著性差异(P>0.05)。结论:在氟中毒引起氟斑牙的过程中,大鼠切牙成釉细胞发生明显的DNA损伤。
Objective：This study was conducted to explore the effects of sodium fluoride （NaF）on DNA damage in rat incisor ameloblasts.Method：Male SD rats were provided with distilled water containing NaF at the doses of 10,50 and 100 mg/L respectively for 120 days.The rat model for dental fluorosis was made.DNA damage was measured with single cell gel electrophoresis（or comet assay）.Result：NaF at the doses of 50 and 100 mg/L for 120 d could increase serum fluoride concentration significantly,statistics analysis yielded close relationship between the dose of NaF in water and the level of NaF in serum （relative coefficient was 0.9153,P 0.05）.NaF at the doses of 10,50 and 100 mg/L could cause dental fluorosis at rat mandibular incisor obviously.The comet cell length was more than that of control at the dose of 50 mg/L NaF （P 0.05）.Compared with control,the comet cell length,Olive tail moment and tail distribution moment at the group of 100 mg/L NaF were increased significantly （P 0.05）.But fluoride at the dose of 10 mg/L NaF failed to induce DNA damage in incisor ameloblasts obviously （P 0.05）.Conclusion：Fluoride at the doses of 10,50 and 100 mg/L NaF could induce dental fluorosis in rat mandibular incisor obviously and at relative higher doses such as 50,100 mg/L NaF could cause DNA damage significantly in incisor ameloblasts.It was suggested that DNA damage play important role in the formation of dental fluorosis induced by fluoride.
出处
《临床口腔医学杂志》
2010年第7期403-405,共3页
Journal of Clinical Stomatology
基金
广东省医学科研基金资助项目(A2008331)
广东省自然科学基金资助项目(9151022401000003)
广东药学院师资队伍建设经费资助
关键词
氟
氟斑牙
DNA损伤
fluoride
dental fluorosis
DNA damage
