摘要
目的 建立红细胞胆固醇荧光测定法.方法 红细胞溶血后血液中的胆固醇、胆固醇酯与胆固醇酯酶（CEH）、胆固醇氧化酶（CHOD）作用生成△4-胆甾烯酮和过氧化氢（H2O2）,H2O2在辣根过氧化物酶（HRP）的催化下使底物酪氨酸氧化,生成具有荧光的酪氨酸二聚体,荧光强度与胆固醇的浓度成正比,在标准曲线上查出或求出全血胆固醇浓度.经过（全血TC-血浆TC）/红细胞压积＋血浆TC,计算出红细胞胆固醇浓度.结果 用此方法检测98例样本红细胞TC ±s,mmol/L）：包括健康组40例,其中男性20例：4.63±0.56,女性20例：3.82±0.55;冠心病组18例：5.55±0.57;高脂血症40例：4.55±0.47.精密度：批内CV2.7%,批间CV3.2%.回收率96.1%～99.3%,平均97.5%.结论 成功建立了红细胞胆固醇荧光测定法,并在临床进行了初步应用,证实采用检测底物的荧光强度对胆固醇定量,具有较高精密度,微量快速,简便易行,重复性好,有着较好的临床适应性.
Objective To establish a red blood cell cholesterol fluorescence. Methods Hemolysis after blood cholesterol, cholesterol esters and cholesterol esterase （CEH）,cholesterol oxidase （CHOD） reacts A 4-Cholesteric Ketene and hydrogen peroxide （H2O2） ,H2O2 in the horseradish peroxidase （HRP） to catalyze the oxidation of tyrosine to produce a fluorescent dimer of tyrosine,the fluorescence intensity proportional to the concentration of cholesterol in the calibration curve or calculate the concentration of blood cholesterol. Through the （whole blood TC-plasma TC）/hematocrit and plasma＋TC, calculated red blood cholesterol levels. The results with this sample of red blood cells were detected 98 cases of TC （ x± s, mmol/L） ：including health group of 40 cases, of which 20 males ： 4.63 ±0.56, femininity 20 ： 3.82 ±0. 55 ; coronary heart disease group, 18 patients： 5.55 ± 0.57; hyperlipemia 40 cases： 4.55 ± 0. 47. Precision： The intra CV2.7% and inter CV3.2%. Recovery 96.1%±99.3%,average 97.5%. Conclusion The establishment of a red blood cell cholesterol fluorescence spectrometry,and a preliminary application in clinical ,confirmed by detection of the fluorescence intensity of substrate quantification of cholesterol,high precision,micro quick,simple and reproducible,with good clinical adaptation.
出处
《现代检验医学杂志》
CAS
2010年第5期54-55,58,共3页
Journal of Modern Laboratory Medicine
基金
陕西省卫生厅科研基金资助项目（2010H08）
关键词
红细胞胆固醇
酪氨酸
酪氨酸二聚体
荧光测定
erythrocyte cholesterol
tyrosine
tyrosine dimers
fluorescence determination
