摘要
目的:比较自身免疫性肝炎(autoimmune hepatitis,AIH)患者与健康对照者(healthy controls,HCs)外周血CD4+CD25+调节性T细胞(CD4+CD25+Tregs)数量、免疫抑制功能的变化,探讨CD4+CD25+Tregs参与AIH发病的可能机制。方法:采用流式细胞仪检测8例AIH患者及15例健康对照组的外周血CD4+CD25+Tregs数量的百分比及绝对数量;采用共同培养方法检测AIH患者外周血CD4+CD25+Tregs的免疫抑制功能的变化;实时荧光定量聚合酶链反应(RT-FQ-PCR)检测AIH患者外周血CD4+CD25+Tregs中FoxP3mRNA的表达。结果:AIH患者外周血CD4+CD25+Tregs数量明显低于HCs(p<0.01);混合淋巴细胞共同培养结果显示,AIH患者外周血CD4+CD25+Tregs抑制功能明显低于HCs组(p<0.01);AIH患者外周血CD4+CD25+Tregs的FoxP3mRNA相对表达量显著降低,与HCs组比较有显著性差异(p<0.01)。结论:CD4+CD25+Tregs细胞的数量的减少和Foxp3表达的降低所造成的CD4+CD25+Tregs细胞免疫抑制功能受损可能是AIH发病的一个因素。
Objective：To analyze the number,suppressive function of CD4 ＋ CD25 ＋ regulatory T cells（CD4 ＋ CD25 ＋ Tregs）in pe-ripheral blood from patients with autoimmune hepatitis（AIH）and healthy controls（HCs）and research the possible mechanisms that CD4 ＋ CD25 ＋ Tregs participate in the onset of AIH.Methods：Flow cytometry was used to analyze the frequency of CD4 ＋ CD25 ＋ Tregs from 8 patients with AIH and 15 healthy controls;Functional characterization of CD4 ＋ CD25 ＋ Tregs from the peripheral blood mononu-clear cells（PBMC）of patients and HCs were analyzed by suppression of proliferation by co-cultured effector CD4 ＋ CD25 ＋ T cells.Foxp3 message（mRNA）expression level was assessed by quantitative real-time polymerase chain reaction.Results：The proportion of CD4 ＋ CD25 ＋ Tregs from the patients with AIH was significantly decreased as compared with the healthy controls（P0.01）;CD4 ＋ CD25 ＋ Tregs from patients with AIH exhibited hampered inhibition of responder CD4 ＋ CD25-cell proliferation compared with those from healthy controls（P0.01）;CD4 ＋ CD25 ＋ Tregs from patients with AIH expressed lower levels of FoxP3 mRNA.Conclusions：Decreased amount of CD4 ＋ CD25 ＋ Tregs and decreased expression of Foxp3 impair the suppressive activity of CD4 ＋ CD25 ＋ Tregs,which may be a factor in the pathogenesis of AIH.
出处
《现代生物医学进展》
CAS
2010年第18期3447-3450,共4页
Progress in Modern Biomedicine
基金
黑龙江省卫生厅基金资助(2007-278)
