摘要
目的:探讨微柱凝胶技术在新生儿溶血病(HDN)筛查中的应用以及结果分析。方法:采用微柱凝胶技术对633例疑似HDN患儿进行检测,用经典抗球蛋白法做对照。结果:633例筛查实验微柱凝胶技术阳性率为30.5%,28%为ABO-HDN,2.5%为RH-HDN,其中直接抗球蛋白实验阳性率为6.8%,患儿红细胞抗体放散实验阳性率为30.5%(抗A抗体阳性89例,抗B抗体阳性45例,抗AB抗体阳性43例,抗D抗体阳性10例,抗E抗体阳性6例),血清游离抗体鉴定阳性率为13%,而经典抗球蛋白法的直接抗球蛋白实验阳性率为3%,患儿红细胞抗体放散实验阳性率为仅为24.7%。结论:在温州地区的HDN以ABO-HDN最为常见,抗体分布特征为抗A抗体>抗B抗体>抗AB抗体>抗D抗体>抗E抗体。新生儿溶血微柱凝胶技术能快速准确检测出HDN,具有很高的灵敏度,并能够检出经典抗球蛋白法所不能检出的阴性标本,是直观可靠的方法。
Objective：To investigate the application of microcolumn gel technique in screening hemolytic disease of the newborn （HDN） and results analysis.Method：633 newborns with suspicious HDN were tested by microcolumn gel technique,compared with traditional tube agglutination technique （TAT） test.Result：Among 633 cases of suspicious HDN,the positive rate of HDN screening test was 30.5%,and 28% were ABO HDN,2.5% were RH HDN,from which the positive rate of direct Coombs＇test was 6.8%,elution test was 30.5%（ 89 cases of anti-A,45 cases of anti-B,43 cases of anti-AB,10 cases of anti-D,6 cases of anti-E）,and serum free antibody rate was 13%,.while the rates of direct Coombs＇test and elution test results of traditional TAT anti human IgG test were 3% and 24.7%,respectively.Conclusion：ABO-HDN was common in Wenzhou region,the distribution characteristics of antibody was anti-Aanti-Banti-ABanti-Danti-E.Compared with the traditional TAT test,microcolumn gel technique would be more sensitive,simple and accurate,which could detect the negative case which couldn＇t be tested by TAT,so microcolumn gel technique would be a dependable method.
出处
《临床血液学杂志（输血与检验）》
CAS
2010年第4期451-453,共3页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
关键词
微柱凝胶技术
新生儿溶血病
hemolytic disease of the newborn
microcolumn gel technique
