摘要
利用黑曲霉作为出发菌株,以提油后的麻疯树饼粕作为培养基,采用固态发酵方式生产蛋白酶。控制培养基湿度为50%,添加5%的葡萄糖作为碳源,在此条件下发酵4d,蛋白酶产量达到最大值(9204U/g)。酶学性质研究表明,蛋白酶最适作用pH为4.0,最适催化温度为50°C,最大催化速度Vmax为18.05μg/min,Km值为2.23mg/mL,有机溶剂对酶活力有明显促进作用,15%(体积分数)甲醇和10%(体积分数)乙醇可以使酶活力分别提高31.21%和24.11%。
Solid-state fermentation was employed to produce protease by Aspergillus niger using deoiled Jatropha curcas seed cake as substrate. The optimal conditions of protease production were studied. Maximum protease production（9 204 U/g of dry substrate, U/g） was obtained at 50 % substrate moisture, a growth period of 4 days, supplementation with 5 % （wt） of glucose. The results of protease analysis showed that the optimal temperature and pH were 50 ℃ and 4.0, respectively. The Vmax and Km of protease produced by Aspergillus niger were 18.05 μg/min and 2.23 mg/mL, respectively. Organic solvent can enhance protease activity. In comparison with control, the activities were increased by 31.21 % and 24.11 % when the protease was solved by 15 % （v/v） of methanol and 10 %（v/v） of ethanol, respectively.
出处
《食品研究与开发》
CAS
北大核心
2010年第10期164-168,共5页
Food Research and Development
基金
教育部"春晖"计划(Z2007-1-52017)
贵州省科学技术基金(黔科合J字[2007]2035号)
国家大学生创新性实验计划(贵大国创字2008017号)
贵州大学大学生创新性实验计划
关键词
麻疯树饼粕
黑曲霉
蛋白酶
固态发酵
Jatropha curcas seed cake （JCSC）
Aspergillus niger
protease
solid-state fermentation
