摘要
目的对牙周组织工程的种子细胞和其体内移植方法进行综述。方法查阅国内外近年来关于细胞治疗在牙周组织工程应用的文献,对牙周组织工程种子细胞及其体内移植的方法进行总结。结果 MSCs是牙周种子细胞的重要来源,其中牙周膜干细胞是首选。BMSCs虽具有很强的促进牙周组织再生的潜力,但应用受到一定限制,而脂肪来源干细胞可作为一种极有前景的替代细胞。干细胞体内移植的方法均能不同程度地促进牙周组织再生,细胞悬液注射法只在促进少量牙龈缺损修复时有效;组织工程支架材料更适用于牙周组织的再生,但尚需进一步改进;而细胞膜片技术无需支架,能实现大量细胞的有序移植,能在不同条件下促进牙骨质、牙周韧带和牙槽骨的再生。结论具有多向分化潜能的干细胞更适宜作为牙周组织工程的种子细胞,其体内移植方法的不断改进,也将进一步促进牙周组织的完全再生。
Objective To introduce the cells and cell-transplantation methods for periodontal tissue engineering. Methods Recent literature about application of cell-based therapy in periodontal tissue engineering was extensively reviewed, the cells and cell-transplantation methods were investigated. Results Mesenchymal stem cells were important cell resources for periodontal tissue engineering, among which peridontal ligament stem cells were preferred. Bone marrow mesenchymal stem cells had several disadvantages in clinical application, and adipose-derived stem cells might be a promising alternative; different transplantation methods could all promote periodontal regeneration to some extent. Single-cell suspension injection could only promote a little gingival regeneration, and tissue engineered scaffolds still needed some improvement to be used in periodontal regeneration, while cell sheet technique, with great cell loading ability and no need of scaffolds, could promote regeneration of cementum, periodontal ligament, and alveolar bone under different conditions. Conclusion Multipotent stem cells are fit to be used in periodontal tissue engineering; improvement of cell-transplantation methods will further promote periodontal regeneration.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2010年第10期1249-1252,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
组织工程
牙周组织
再生
种子细胞
细胞移植
Tissue engineering Periodontium Regeneration Seeding cells Cell transplantation
