摘要
【目的】观察大鼠脓毒血症时有核细胞过氧化物酶体增殖物激活受体γ(PPARγ)活性、血浆促炎介质TNF-α及抗炎介质IL-4的变化,探讨其关系。【方法】雄性SPF级SD大鼠90只,体质量280～300g,随机分为正常对照组、假手术组、脓毒血症组,每组再分12h、24h、48h三亚组,每组10只。采用盲肠结扎穿孔(CLP)制作大鼠脓毒血症模型,ELASA方法测定有核细胞的PPARγ活性及血浆中IL-4的水平变化趋势。【结果】脓毒血症组大鼠血浆促炎介质TNF-α与正常对照组和假手术组相比升高明显,分别为(162±32vs31±7,32±8)pg/mL,随时间延长有上升趋势,在术后24h达高峰,后逐渐下降,但仍然处于较高水平状态。抗炎介质IL-4水平也明显较正常对照组和假手术组升高(分别为89±25vs41±7,41±7)pg/mL,并呈持续性升高(70±5,75±8,122±11)pg/mL,存在促炎和抗炎平衡失调。脓毒血症组大鼠有核细胞PPARγ活性与正常对照组和假手术组相比明显降低,分别为(0.263±0.017,vs0.292±0.005,0.294±0.007)并随病情加重而逐渐下降。脓毒血症组大鼠有核细胞PPARγ活性与血浆促炎介质TNF-α水平呈负相关,r=-0.655,P=0.040。【结论】脓毒血症大鼠存在炎症-抗炎系统失衡,有核细胞PPARγ活性下降,且与血浆促炎介质TNF-α呈负相关。
Objective To observe the relationship between the peroxisome proliferator activated receptor γ（PPARγ） activity in nucleated cells and pro-inflammatory mediator tumor necrosis factor α（TNF-α）,anti-inflammatory mediator IL-4 in plasma of sepsis rats.Methods Ninety male SD rats weighing 280-300 g at specific pathogen free（SPF） level were randomly divided into three groups,which were control group,sham operation group,and sepsis group.And every group was further divided into 12 h,24 h,and 48 h subgroups,every subgroup containing 10 rats.Sepsis model of rats were established by the operation of caecal ligation and puncture.The PPARγ activity in nucleated cells and pro-inflammatory mediator TNF-α,anti-inflammatory mediator IL4 in plasma were detected by ELASA.Results Compared with control group and sham operation group,the TNF-α level was increased in sepsis group（162 ± 32 pg /mL vs 31 ± 7 pg /mL and 32 ± 8 pg /mL,respectively）,and the peak level appeared in 24 h.Compared with control group and sham operation group,the IL-4 level was increased in sepsis group（89 ± 25 pg /mL vs 41 ± 7 pg /mL and 41 ± 7 pg /mL,respectively） and the increment was maintained during the experiment（70 ± 5 pg /mL;75 ± 8 pg /mL;122 ± 11 pg /mL）.The PPARγ activity in nucleated cells in sepsis group were decreased（0.263 ± 0.017 vs 0.292 ± 0.005 and 0.294 ± 0.007,respectively）,and correlated to the severity of pathogenic condition.There was negative correlation between pro-inflammatory mediator TNF-α and the PPARγ activity in nucleated cells in sepsis group,r =-0.655,P = 0.040.Conclusion In sepsis rats,the pro-inflammatory mediator TNF-α and anti-inflammatory mediator IL-4 in plasma were both increased.The PPARγ activity in nucleated cells was decreased,and there was negative correlation between TNF-α and PPARγ activity.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2010年第5期630-634,640,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2008B030301057)
关键词
过氧化物酶体增殖物激活受体
炎症介质
脓毒血症
peroxisome proliferators activated receptors
mediators of inflammation
sepsis
