摘要
建立了LC-MS/MS法测定性保健品中可能违禁添加的西地那非、他达那非、甲磺酸酚妥拉明、红地那非、盐酸伐地那非、还阳酚和还阳碱7种药物。采用C18色谱柱,以0.02%甲酸-乙腈为流动相,梯度洗脱,以D3-他达那非为内标,上述7种药物分别在0.1～10、0.3～30、0.015～1.5、0.05～5、0.4～40、0.25～25和1～100μg/ml浓度范围内线性关系良好,回收率为85.29%～119.5%,RSD均小于5%。
A LC-MS/MS method was established for the determination of sildenafil,tadalafil,phentolamine mesylate,acetildenafil,vardenafil hydrochloride,Huanyangfen and Huanyangjian added illegally in sex-health products.A C18 column was used with the mobile phase of 0.02% formic acid-acetonitrile by gradient elution.D3-Tadalafil was used as the internal standard.The calibration curves for these compounds were linear in the concentration ranges of 0.1 10,0.3-30,0.015-1.5,0.05-5,0.4-40,0.25-25 and 1-100 μg/ml,respectively.The recoveries were 85.29% 119.5%,with RSDs less than 5%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2010年第10期755-758,共4页
Chinese Journal of Pharmaceuticals
