摘要
建立同时测定减肥保健食品中4种利尿剂(吲达帕胺、布美他尼、托拉沙米、氨苯喋啶)的超高效液相色谱-电喷雾串联质谱分析方法。不同类型的减肥保健食品经甲醇(含1%冰乙酸)超声提取后,以ACQUITY UPLC HSS T3(1.8μm,100 mm×2.1 mm)分离,进行UPLC/MS/MS多反应监测模式下的定性及定量分析。咖啡、茶叶、胶囊和片剂中4种利尿剂的检出限为(0.3～7.0)mg/kg;减肥口服液中4种利尿剂的检出限为(0.3～2.5)μg/L。在低、中、高的三个添加水平范围内的平均回收率为80.0%～105.1%;日内精密度均小于8%,日间精密度均小于10%。该方法分析速度快,灵敏度高,重现性好,可用于减肥保健食品的实际检验工作。
A comprehensive based on ultra performance liquid chromatography tandem mass spectrometry has been developed for the simutaneous determination of 4 diuretics（triamterene,bumetanide,indapamide,torasemide）.After various weight-loss functional foods were extracted with methnal（1% acetic acid） under ultrasoniccation.Qualitative and quantitative analysis was carried out for the analyte under the MRM mode after the chromatographic separation on Waters ACQUITY UPLC HSS T3（1.8 μm,100 mm×2.1 mm） column.The limits of detection for 4 diuretics in tea,coffee,capsule and table were（0.3～7.0） mg/kg and the limits of detection for 4 diuretics in solvent was（0.3～7.0） mg/kg.The mean recoveries at the three spiked levels were 80.0%～105.1%,with the intra-day precision less than 8% and the inter-day precision less than 10%.The method is accurate,sensitive,simple,and adapts to the determination of 4 diuretics in the different weight-loss functional foods.
出处
《食品工业》
北大核心
2010年第5期88-91,共4页
The Food Industry
