摘要
目的改进了比伐卢定氨基酸组成比测定方法。方法采用C18(250 mm×4.6 mm,5μm)色谱柱;流动相A为磷酸盐缓冲液,流动相B为60%乙腈,梯度洗脱;柱温:37℃;流速:1.4 mL/min;检测波长为248 nm。结果各氨基酸回收率为95.1%～98.3%,RSD为0.8%～1.2%。结论此法简便灵敏,结果准确可靠,可用于比伐卢定氨基酸组成比的测定。
Purpose To improve the method for the determination of amino acids in Bivalirudin.Methods The C18（250 mm×4.6 mm,5 μm） column was used.The phosphate buffer was used as mobile phase A,60% acetonitrile was used as mobile phase B and the temperature of column was 37 ℃.The flow rate was 1.4 mL/min and the detective wavelength was 248 nm.Results The average recovery was 95.1%～98.3% and RSD was 0.8%～1.2%.Conclusion The method is simple,sensitive and accurate.It can be used for the determination of amino acids in Bivalirudin.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2010年第5期338-340,共3页
Chinese Journal of Biochemical Pharmaceutics
