摘要
在pH 3.0~3.8的BR缓冲溶液中,偶氮胂Ⅲ-Ba（Ⅱ）与蛋白质结合形成复合物,导致体系的共振散射信号增强,据此建立了蛋白质测定的共振散射光谱法。结果表明,在优化的实验条件下,牛血清白蛋白（BSA）的线性范围为0~1.0 mg/L,检出限为21.4μg/L;将该法用于人血清中蛋白质的测定,与医院测定结果基本一致;方法的批内精密度为2.8%（n=6）,批间精密度为3.5%（n=6）,回收率为96.0%~100.0%。
In a medium of britton-robinson buffer（pH 3.0~3.8）,a compound was formed between Arsenazo Ⅲ-Ba（Ⅱ）and proteins,which lead to great enhancement of the reonacence light scattering singal of ArsenazoⅢ-Ba（Ⅱ）.Based on this,a novel resonance light scattering method was developed for the determination of proteins in aqueous solution.Under optimal conditions,the linear range of calibration curve for the determination of bovine serum albumin（BSA）was 0~1.0 mg/L,the detection limit was 21.4 μg/L.The method has been applied to the determination of total protein in human serum samples and the results were agreement with those reported by the hospital.The precision was tested at optimum condition.Intra-CV=2.8%（n=6）,inter-CV=3.5%（n=6）and the recovery was in the range of 96.0%~100.0%.
出处
《广东微量元素科学》
CAS
2010年第9期29-33,共5页
Trace Elements Science
基金
浙江省分析测试基金项目(2008F70051)
