摘要
肌肉生长抑制素是控制动物骨骼肌生长发育的重要细胞因子,采用PCR-SSCP和测序方法分析了150尾大黄鱼样本肌肉生长抑制素基因(myostatin)外显子Ⅱ的单核苷酸多态性,结果发现,外显子Ⅱ中有10个多态位点,分别是29、131、206、261、231、243、265、273、297和300位。其中131位点T→A突变引起相应的氨基酸由L→Q,265位点T→C突变引起相应氨基酸由C→R,261位点A→G突变引起相应氨基酸由S→G,231位点的T→C突变引起相应氨基酸由W→S,273位点的C→T突变引起相应的氨基酸由R→W,297位点的A→G突变引起相应氨基酸由I→V,243位点的G→A突变引起相应氨基酸由D→N,300位点的G→A突变引起相应氨基酸由E→K。29位点A→G和206位点C→A均属于同义突变。存在6种基因型,他们的基因频率分别是0.57、0.23、0.10、0.04、0.02、0.04,该基因位点的杂合度为0.33。
Myostatin（MSTN） as an important cytokine controls the growth and development of skeletal muscle in animals.Ten single nucleotide polymorphisms（SNPs） were totally identified in the exon Ⅱof MSTN gene from 150（70 of one-year old and 80 of two-year old） large yellow croakers,Pseudosciaena crocea,individuals by PCR-SSCP and sequencing.The ten polymorphic sites were located at 29（A→G）,131（T→A）,206（C→A）,231（T→C）,243（G→A）,261（A→G）,265（T→C）,273（C→T）,297（A→G） and 300（G→A）.Base mutations at sites 131,265,261,231,273,297,243 and 300 resulted in amino acid substitutions of L→Q,C→R,S→G,W→S,R→W,T→V,D→N and E→K,respectively.However,base mutations at sites 29 and 206 did not cause amino acid substitution.There were six genotypes in the examined population,whose frequency was 0.57、0.23、0.10、0.04、0.02and 0.04,respectively.The heterozygosity of MSTN locus was 0.33.
出处
《海洋通报》
CAS
CSCD
北大核心
2010年第5期554-559,共6页
Marine Science Bulletin
基金
国家863项目(2006AA10A405)
国家自然科学基金(30871916)
