摘要
采用SDS抽提法、TRIzol法、CTAB-LiCl、DEPC水法对羊肚菌菌丝体进行了总RNA的提取,并对其进行了紫外及琼脂糖凝胶电泳检测。结果表明:4种提取方法中DEPC水法提取的总RNA质量最好,SDS抽提法次之,CTAB-LiCl法和TRIzol法效果最差。从RNA质量、操作简单、经济与否等方面综合分析,DEPC水法是羊肚菌菌丝体总RNA提取较为理想的方法。
The main object of this paper was to study total RNA extracted from Morchella mycelium by the 4 methods including SDS method,TRIzol method,CTAB-LiCl method and DEPC water method and to carry out comparative analysis of the method and to carry out comparative analysis of the eletrophoretic results and purity of total RNA.The results showed that the purity and integrality of extracted total RNA was as following：DEPC water methodSDS methodCTAB-LiCl methodTRIzol method.Comprehensive analysis from the RNA quality,operation,economic and so on,DEPC water was the best one of extracting total RNA from Morchella mycelium.
出处
《北方园艺》
CAS
北大核心
2010年第20期150-151,共2页
Northern Horticulture
基金
陕西省教育厅资助项目(09JK822)
延安大学大学生科技创新训练资助项目(YD2009-209)
关键词
羊肚菌
总RNA
提取方法
Morchella
total RNA
extraction method
