摘要
目的:比较3种高脂血症大鼠的造模方法,为实验研究选择最佳高脂血症动物模型提供依据。方法:40只SD大鼠随机分为普通饲料喂养(A组)、植物油高脂饲料喂养(B组)、猪油高脂饲料喂养(C组)及脂肪乳剂灌胃喂养(D组)各10只。1个月后比较3组大鼠的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)及肝脏形态学改变。结果:与A组比较,TC在B、C、D组均明显升高;TG在C组明显升高;HDL-C在C组明显下降,D组则明显上升;LDL-C在B、C、D组均明显升高(均P<0.05,0.01)。结论:综合以上各组大鼠4个指标的升降情况,以猪油固体饲料喂养的大鼠最符合高脂血症诊断标准,且比较接近人类饮食成分及方式,造模效果理想。
Objective： To compare three kinds of experimental hyperlipidemia rat models.Methods： Forty rats were randomly divided into normal diet group（A）,high fat diet（vegetable oil） group（B）,high fat diet（lard oil） group（C） and group of intragastric administration of fat emulsion（D）,10 rats in each group.One month later,the total cholesterol（TC）,triglyceride（TG）,high density lipoprotein-cholesterol（HDL-C）,low density lipoprotein-cholesterol（LDL-C）,and morphological changes of the liver were compared among the groups.Results：（1） As compared with group A,TC was increased in groups B,D and C,TG was significantly increased and HDL-C significantly reduced in group C,HDL-C significantly increased,and LDL-C in groups B,C and D was significantly increased（P〈0.05,0.01）.Conclusion：The hyperlipidemia models in rats fed on the lard solid met the diagnostic standards of hyperlipidemia.
出处
《中国康复》
2010年第5期330-331,共2页
Chinese Journal of Rehabilitation
基金
国家自然科学基金项目(30873309)
关键词
高脂血症模型
猪油
植物油
脂肪乳剂
hyperlipidemia models in rats
vegetable oil
lard oil
gastric perfusionint ralipid
