摘要
目的探讨家兔气腹模型中不同麻醉药物对肺的损伤情况。方法以50只家兔为研究对象,按照麻醉药物不同随机分为丙泊酚1组(P1组)、丙泊酚2组(P2组)、戊巴比妥钠组(Pen组)、咪达唑仑+芬太尼组(M+F组)、氯胺酮组(K组),每组10只。麻醉后除P1组为假气腹组外,其他组建立气腹,持续3h后解除气腹,20 min后取各组血浆测血浆蛋白含量,分别取肺组织称取湿干重计算肺组织含水量、测定髓过氧化物酶(myleopemxidase,MPO)含量,取支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)离心后测定上清液蛋白含量和沉渣中白细胞,计算肺通透系数(lung permeability index,LPI)。结果 Pen组的麻醉死亡率明显高于其他组。肺组织含水量、LPI、BALF中白细胞数及肺组织MPO活力方面,四组之间无差异(P>0.05),均明显大于P1组(P<0.01)。结论家兔戊巴比妥钠静脉麻醉的麻醉风险明显增大,死亡率远远高于其他各种麻醉方法;3h的持续气腹造成了肺组织含水量、LPI、BALF中白细胞数及肺组织MPO活力方面的明显变化,但四种不同的麻醉用药方法没有对家兔肺损伤情况造成差异。
Objective To evaluate the effect of different anaesthetics on lung injury in rabbits subjected to pneumoperitoneum. Methods Fifty rabbits were randomly assigned to following groups according to different anaeathetics. Group （ P1 ） and group （P2） received propofol, group （Pen） received pentobarbital sodium, group （ M ＋ F ）received midazolam and fentanyl,group （K） received ketamine. Group （P1） was subjected to a sham pnettmoperlto- neum and the other rabbits were subjected to 3 hours pneumoperitoneum. After 20 rain of desuftlation subsequent to cessation of pneumopefitoneum, blood, lung, and bronchoalveolar lavage fluid （BALF） samples were obtained,white blood cell（WBC） count in BALF were determined. The myleopemxidase （MPO） activity and wet to dry weight ratio （W/D） were also measured. Results The mortality rate of group （Pen） was the highest in all groups （P 〈 0. 01 ）. Lung permeability index（ LPI）, WBC count in BALF and the lung tissue MPO activity had no difference in either two groups （P 〉 O. 05） except group （ P1 ） （P 〈 0. 01 ）. Conclusion It seemed that pentobarbital sodium as an anaesthet- ics by intravenous injection is not suitable for rabbit compared with the other three anaeathetics, but there＇ s no difference in the effect on lung injury among the four groups.
出处
《中国临床新医学》
2010年第10期929-932,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
广西青年科学基金项目(编号:桂科青0728041)
关键词
麻醉药
气腹
肺损伤
肺通透系数
家兔
Anaesthetics
Pneumoperitoneum
Lung injury
Lung permeability index
Rabbit
