摘要
目的:评价CHROMager念珠菌显色培养基在念珠菌深部感染标本检测中的应用价值。方法:用CHROMager念珠菌显色培养基分离和鉴定来源于贵州省内不同地区224例深部感染患者的真菌标本,采用PCR扩增和DNA序列分析方法检测分离真菌rDNA的ITS序列。结果:224例深部感染患者标本共分离念珠菌243株,显色培养基鉴定16例标本为混合念珠菌感染,占7.1%,其中155株白假丝酵母菌63.8%,居分离率首位;显色培养基鉴定结果与基因鉴定结果符合率为97.7%。结论:深部感染念珠菌以白假丝酵母菌最常见,CHROMager念珠菌显色培养基能快速、简便、准确地分离临床常见的念珠菌,并可有效地提高混合念珠菌感染的检出率。
Objective： To evaluate CHROMager candida medium（CCM） in identification of pathogenic Candida from patients with deep infection,and to invastigate the microflora and distribution of Candida in Guizhou area.Methods： Two hundred and twenty-four samples derived from patients with deep infectious diseases were cultivated and indentified on CCM.ITS of strains isolated were amplified and determined by PCR and DNA sequencing.Results： Two hundred and forty-three strains of fungi including 155 C.albicans（63.8%） were isolated from the 224 samples with CCM.Sixteen samples（7.1%） were proved multi-fungi infection.The coincidence rate of identification results of CCM method with those of gene analysis method was 97.7%.Conclusions： C.albicans is come in Candida caused deep infection in Guizhou.CCM culture is a quick and convenient method for identification of Candida in clinic,and is especially useful in detecting mixed multi-fungi infection.
出处
《贵阳医学院学报》
CAS
2010年第5期462-464,共3页
Journal of Guiyang Medical College
基金
贵阳市云岩区科技基金[云科技合同字(2008)第1-7号]
贵州省教育厅科技基金(黔教科20090136)资助项目
关键词
培养基
念珠菌属
感染
culture medium
candida
infection
