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LC-MS分析生物样品中肽类及蛋白类药物的技术问题及其解决方案 被引量：8

Common issues and counterplans on LC-MS analysis of peptides and proteins in bio-samples
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摘要 肽类及蛋白类药物的理化性质较为特殊,其LC-MS分析方法与传统的化学药物相比,存在不少特殊性,在定量分析测定上也存在不少问题。这些问题包括:容器壁对肽类及蛋白的吸附作用、生物样品中肽类及蛋白易降解、生物样品基质中大量内源性肽类及蛋白对测定的干扰、极性较大在反相柱上不易保留、质谱分析肽类及蛋白时对离子源和质量分析器的要求以及内标不易寻找等。本文对液相色谱-质谱联用技术在肽类及蛋白类样品分析中的常见问题及其解决方法进行了综述。 The analytical methods for peptide and protein drugs in the bio-samples using liquid chromatography-mass spectrometry（LC-MS） are different from those for small molecular drugs.Quantification of peptide and protein drugs is faced with many challenges because of their special physicochemical properties,such as the adsorption to solid surfaces,chemical unstable in the bio-samples,interference from endogenous substances,poor retention behaviors,special requirements on ionization sources,mass analyzers and internal standards.This paper focuses on the common issues and counterplans on the LC-MS analysis of peptides and proteins in bio-samples.
作者 周娜 王晶 丁黎
机构地区 中国药科大学药物分析教研室 中国药科大学药物质量与安全预警教育部重点实验室
出处 《中国药科大学学报》 CAS CSCD 北大核心 2010年第5期401-407,共7页 Journal of China Pharmaceutical University
关键词 生物样品 肽 蛋白 液相色谱-质谱联用技术 吸附 衍生化 多电荷离子 bio-sample peptide protein LC-MS adsorption derivatization multiple-charged ion
分类号 R927.2 [医药卫生—药学]
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参考文献56

  • 1Schellenberger V,Wang CW,Geething NC,et al. A recombinant polypeptide extends the in vivo half-life of peptides and proteins in a tunable manner[J]. Nat Biotech,2009,27 ( 12 ) : 1 186 - 1 190.
  • 2Shechter Y, Mironchik M, Saul A,et al. New technologies to prolong life-time of peptide and protein drugs in vivo[J], Int J Pep Thera,2006,13 ( 1 ) : 105 - 117.
  • 3Biron E, Chatterjee J, Ovadia O, et al. Improving oral bioavailability of peptides by multiple N-methylation: somatostatin analogues[J]. Angew Chem Int Ed,2008,47(14) :2 595 -2 599.
  • 4Xu Y, Musson DG. Reversed-phase chiral liquid chromatography on polysaccharide-based stationary phase coupled with tandem mass spectrometry for sinmhaneous determination of four stereoisomers of MK-0974 in human plasma [ J ]. J Chromatogr B, 2008,873 ( 2 ) : 195 - 202.
  • 5Kota U, Chien KY, Goshe MB, et al. Methods in Molecular Biology[ M ]. New York : Humana Press,2009 : 303 - 321.
  • 6Broek I, Sparidans RW, Schellens JH,et al. Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry [ J ]. J Chromatogr B, 2008, 872 ( 1/ 2) : 1 -22.
  • 7Li HY,Rose MJ,Tran L,et al. Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LC-MS-MS [ J ]. J Pharm Tox, 2009,59 ( 3 ) : 171 - 180.
  • 8Che JJ,Meng QF,Chen ZH,et al. Quantitative analysis of a novel HIV fusion inhibitor (sifuvirtide) in HIV infected human plasma using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry [ J ]. J Pharm Biomed Anal,2010,51 (4) :927 - 933.
  • 9Hu XY, Kamberi M. Quantification of apolipoprotein A-I mimetic peptide D-4F in rabbit plasma by high-performance liquid chro- matography coupled with electrospray ionization tandem mass spectrometry[ J]. J Chromatogr B,2009,877 (20/21) : 1 946 - 1 950.
  • 10Togashi Y. Reduced carryover using an HPLC sample injection system with ultrasonic cleaning [ EB/OL ]. ( 2008-06-20 ).http ://www. antpedia. com/? uid-1413 -action-viewspaceitemid-9657.

二级参考文献131

  • 1戴舒佳,廖智,刘秀文,梁宋平,汤仲明.大鼠硬膜外和静脉注射 [^(125)I]虎纹毒素-1后的药代动力学(英文)[J].中国药理学与毒理学杂志,2004,18(5):344-351. 被引量：1
  • 2Hagestam I H, Pinkerton T C. Anal Chem, 1985, 57 (8): 1757.
  • 3Bonfiglio R, King R C, OIah T V, et al. Rapid Commun Mass Spectrom, 1999, 13(12): 1175.
  • 4Xu R N, Fan L, Rieser M J, et al. J Pharm Biomed Anal, 2007, 44(2) : 342.
  • 5Gordien J B, Pigneux A, Vigouroux S, et al. J Pharm Biomed Anal, 2009, 50(5): 932.
  • 6Chambers E, Wagrowski-Diehl D M, Lu Z, et al. J Chromatogr B, 2007, 852(1/2): 22.
  • 7Polson C, Sarkar P, Incledon B, et al. J Chromatogr B, 2003, 785(2) : 263.
  • 8Ridgway K, Lalljie S P D, Smith R M. J Chromatogr A, 2007, 1153(1/2) : 36.
  • 9Wille S M R, Lambert W E E. Anal Bioanal Chem, 2007, 388(7) : 1381.
  • 10Zhou S N, Ouyang G, Pawliszyn J. J Chromatogr A, 2008, 1196/1197(4) : 45.

共引文献19

同被引文献96

  • 1赵建国,彭新君,曾序求.僵蚕提取物中氨基酸种类与含量测定[J].九江医学,2008,23(1):1-2. 被引量：7
  • 2孙会仙,张淑珍,谢剑炜.蛋白多肽类药物的药代动力学分析方法的研究进展[J].国外医学（药学分册）,2006,33(1):63-66. 被引量：7
  • 3张颖.生物样品预处理技术及应用进展[J].天津药学,2006,18(1):56-58. 被引量：11
  • 4Dai Z, Wu Z, Jia S, et al. Analysis of amino acid composition in proteins of animal tissues and foods as pre-column o-phthaldialdehyde derivatives by HPLC with fluorescence detection [J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2014, 3: 1-12.
  • 5Buiarelli F, Gallo V, Di Filippo P, et al. Development of a method for the analysis of underivatized amino acids by liquid chromatography/tandem mass spectrome- try: application on Standard Reference Material 1649a (urban dust)[J]. Talanta, 2013, 115(15): 966-72.
  • 6Petritis KN, Chaimbauh P, Elfakir C ,et al. Ion-pair reversed-phase liquid chromatography for determination of polar underivatized amino acids using perfluorinated carboxylic acids as ion pairing agent [J]. J Chromatogr A, 1999, 833(2): 147-55.
  • 7Kwon JY, Moini M. Analysis of underivatized amino acid mixtures using high performance liquid chromatography/ dual oscillating nebulizer atmospheric pressure microwave induced plasma ionization-mass spectrometry [J]. J Am Soc Mass Spectrom, 2001, 12(1): 117-22.
  • 8Harder U, Koletzko B, Peissner W. Quantification of 22 plasma amino acids combining derivatization and ion- pair LC-MS/MS [J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2011, 879(7-8): 495-504.
  • 9Aruda W, Aruda K. Review of volatile perfiuorocar- boxylic acids as ion pair reagents in LC [J]. Lc gc north america, 2009, 10: 919-27.
  • 10Kotrebai M, Tyson JF, Block E, et al. High-performance liquid chromatography of selenium compounds utilizing perfluotinated carboxylic acid ion-pairing agents and in- ductively coupled plasma and electrospray ionization MS detection[J]. J Chromatogr A, 2000, 866(1): 51-63.

引证文献8

二级引证文献23

中国药科大学学报
2010年 第5期

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