摘要
目的 探讨HBV感染后不同疾病阶段（免疫耐受期、肝炎活动期、非活动携带状态）者断面的树突状细胞（DC）亚群功能和血浆细胞因子水平的差异及其可能的原因.方法 30例HBV感染后不同疾病阶段者,分为免疫耐受组、肝炎活动组和非复制携带组,每组10例;另选10名健康人作为对照组.收集研究对象外周血,检测白细胞介素（IL）-12、IL-10、干扰素（IFN）α、IFN γ、肿瘤坏死因子（TNF）α的表达水平,分析髓样树突状细胞（mDC）和浆细胞样树突状细胞（pDC）的功能（以IL-12含量代表mDC的功能,以IFN α含量代表pDC功能）及其他细胞因子在不同组别间表达水平的差异.细胞因子表达水平的比较,总的HBV感染组与健康对照组比较用t检验,4组间两两比较用秩和检验.结果 HBV感染组IL-12总含量及单个核mDC IL-12含量均低于对照组[分别为（153.12±159.30）pg/ml比（338.48±142.92）pg/m和（9.48±9.57）fg比（15.10±6.25）fg,F值分别为0.225和0.684,P值均＜0.01],两组IFN α总含量及单个核pDC IFN α含量差异无统计学意义[分别为（575.87±477.02）pg/ml比（662.37±356.06）pg/ml和（85.14±68.44）fg比（62.11±627）fg,F值分别为1.467和1.804,P直均＞0.05].组间两两比较结果显示,健康对照组IL-12总含量[（338.48±142.92）pg/ml ]高于其他3组（Z值分别为-3.039、-2.967和-2.949,P值均＜0.01）,单个核mDC IL-12含量[（15.10±6.25）fg]也高于其他3组（Z值分别为-3.027、-2.671和-2.863,P值均＜0.01）;健康对照组IFN α总含量为（662.37±365.06）pg/ml,单个核pDC IFN α含量为（62.11±27.04）fg,均高于肝炎活动组（Z值分别为-3.016和-3.176,P值均＜0.01）.HBV感染不同组别中,仅IFNα水平[分别为（86.17±12.02）pg/ml、（91.76±39.64）pg/ml和（139.32±207.35）pg/ml]高于健康对照组[（53.30±23.92）pg/ml],差异有统计学意义（Z值分别为-2.967、-2.896和-3.054,P值均＜0.01）.结论 HBV感染后,DC功能和IFN α水平存在不同程度的异常,但是HBV不同转归期DC功能无明显差异.DC功能缺陷和IFN α水平异常可能是HBV感染慢性化的原因之一.
Objective We studied the function of dendritic cells subsets and the cytokines levels in plasma in patients with different periods of returning （immune tolerance, hepatitis active and non-replicating period） and aimed to explore the possible reasons for HBV chronic infection. Methods Thirty HBV infected patients in different stages of infection were enrolled and divided into three groups： the immune tolerance group （10 cases）, the hepatitis active group （10 cases）, and the non-replicating group （10 cases）. Ten healthy people were enrolled and served as controls. Blood from the patients and controls were collected and the dendritic cells subsets function （The cytokine levels in different groups） were analyzed using statistical method.Results The total IL-12 output and single nucleus IL-12 output of the total HBV infected patients were lower than that of the healthy control group （P 〈 0.01）. The total IFN α output and single nucleus IFN α output of the total HBV infected patients had no significant difference between the total HBV infected patients and the healthy controls （P 〉 0.05）. The total IL-12 output of the healthy control group was higher than that in others 3 groups of the HBV infected patients. （Z = -3.039, -2.967 ,-2.949, P 〈0.01） and the single nucleus IL-12 output of the healthy control group was also higher than that in others 3 groups of the HBV infected patients. （Z =-3.027, -2.671, -2.863, P 〈0.01）. The total IFN α output and the single nucleus IFN α output of the healthy control group was higher than that in the hepatitis active group of HBV infected patients （Z = -3.016, -3.176, P 〈 0.01）. While the plasma IFN α cytokine levels in the 3 HBV infection groups were higher than in the healthy control group （Z = -2.967, -2.896, -3.054, P 〈 0.01）. Conclusion Difference existed between the function of denritic cells subsets and the IFN α levels in different returning periods of HBV infected patients. The function of denritic cells subsets has no significant difference in HBV patients with different periods of returning. The flawed function of denritic cells subsets and the abnormal IFNα level may be one of the reasons for chronic HBV infection.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2010年第10期731-734,共4页
Chinese Journal of Hepatology
基金
基金项目：北京市科委重大项目（H 020920020690）
国家高技术研究发展计划（863计划）生物和医药技术领域＂重大疾病的分子分型和个体化诊疗＂（2006AA02A410）
