摘要
目的探讨FTY720抑制人卵巢癌细胞系SKOV3生长及诱导凋亡的作用机制。方法不同浓度的FTY720溶液作用人卵巢癌细胞株SKOV3,MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡率及分析细胞周期的变化,Western-blot分析Caspase蛋白的变化。结果 FTY720对SKOV3细胞的生长抑制具有剂量时间依赖性。SKOV3细胞随着FTY720作用时间的延长,凋亡细胞数量增多,G1期细胞比例逐渐上升。Western-blot结果显示Caspase 3,Caspase 9和PARP都受到激活。结论 FTY720可以诱导人卵巢癌细胞株SKOV3发生凋亡,激活内源性Caspase途径和诱导细胞发生G1期阻滞,是FTY720抑制卵巢癌细胞生长作用的可能机制之一。
Objective To investigate the growth-inhibitory and apoptosis-inducing effect of FTY720 on human ovary carcinoma cell line SKOV3 and its mechanism in vitro.Methods We collect SKOV3 cells at 24h and 48h respectively under different concentration of FTY720.The inhibition rates of cells were determined by methyl thiazolyl tetrazolium（MTT） assay to evaluate the effect of FTY720.The percentage of apoptosis and cell cycle progression were detected by flow cytometry（FCM）.Caspase proteins were determined by western-blot.Results Growth inhibition and apoptosis induction were evoked by different concentrations of FTY720 in SKOV3 cells in time-and concentration-dependent manner.FTY720 greatly inhibited human ovary cancer cell SKOV3 proliferation.This effect was associated with G1 phase cell cycle arrest and apoptosis.Western-blot showed that caspase 3,caspase 9 and PARP were activated.Conclusion FTY720 can inhibit the proliferation and induce SKOV3 cells apoptosis.Apoptosis induced by FTY720 may be regulated through G1 phase cell cycle arrest and the activation of endogenous caspase pathway.
出处
《黑龙江医学》
2010年第10期723-726,共4页
Heilongjiang Medical Journal
