摘要
目的:对大孔树脂富集纯化荆芥穗总黄酮的工艺条件及参数进行优化。方法:先以总黄酮比吸附量和解吸率为指标,通过静态吸附与解吸试验对9种树脂型号进行筛选,再通过动态吸附与解吸试验对富集纯化工艺参数进行全面优化。结果:HP-20树脂对荆芥穗总黄酮的吸附与解吸性能较好;确定最佳吸附条件为径高比1∶6,样品溶液pH值为6.0,浓度0.4 g(生药)/mL,吸附流速2 BV/h和最佳吸附量133.76 mg(总黄酮)/g(干树脂);最佳解吸条件为80%乙醇洗脱,洗脱流速4 BV/h和溶剂用量3 BV。结论:HP-20树脂可有效地对荆芥穗总黄酮进行富集纯化。
Objective：To establish an optimum enrichment and purification process of total flavonoids in Schizonepeta tenuifolia by macroporous resins.Methods：9 kinds of resins were compared and the best one was chosen.Then the technique condition for separating and purifying total flavonoids from Schizonepeta tenuifolia was studied.Results：HP-20 was selected for its excellent adsorption and desorption properties,80% ethanol was found to be the best elution solution.The best result was based on the follows： pH value-6.0,feeding rate-2 BV/h,sample concentration-0.4 g/mL,the ratio of total flavonoids to HP-20 macroporous resin-133.76 mg/g,elution flowing rate-4 BV/h,eluting agent amount-3 BV.Then the purity reached 74.31% and the yield was 97.13%.Conclusion：The total flavonoids of Schizonepeta tenuifolia can be effectively purificated and separated by HP-20 macroporous resin.
出处
《中药材》
CAS
CSCD
北大核心
2010年第9期1476-1480,共5页
Journal of Chinese Medicinal Materials
关键词
大孔树脂
荆芥穗
总黄酮
富集纯化
Macroporous resins
Schizonepeta tenuifolia Briq.
Total flavonoids
Enrichment and purification
