摘要
目的了解四川省ＨＩＶ１各亚型的流行情况。方法采集四川省内检出的１２例ＨＩＶ１感染者的全血标本，提取核酸后用套式聚合酶链反应（ＮｅｓｔｅｄＰＣＲ）技术扩增ＨＩＶ１ｅｎｖ基因区，用异源双链电泳泳动法进行ＨＩＶ１亚型的分析。结果１２份标本中有１１份ＰＣＲ扩增阳性，亚型分析证明１１份标本均为Ｂ亚型。结论ＨＩＶ１Ｂ亚型可能是四川省流行的主要亚型。
Objective To investigate the epidemiology of HIV 1 in Sichuan Province.Methods The nuclear acids were extracted from blood samples of 12 infected individuals. The 0.7kb segment of HIV 1 envgene was amplified by nested PCR and the HIV 1 genetic subtypes were then assayed by heteroduplex mobility method.Results Eleven of 12 samples were determined to be positive by PCR amplification and all the 11 samples were found to be of genetic subtype B. Conclusion HIV 1 subtype B may be dominant in Sichuan Province.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第2期94-96,共3页
Chinese Journal of Infectious Diseases
