摘要
建立了苹果和土壤中阿维菌素残留量的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法,并且应用本方法研究产自泰安、西安及北京的苹果和土壤中阿维菌素的代谢规律。样品经乙腈萃取后,用饱和NaCl盐析分层,静置30min后,旋转蒸发浓缩,氨基柱净化,应用UPLC-MS/MS选择多反应监测(MRM)正离子模式扫描测定。本方法最低检出浓度(LOQ)为1.0μg/kg(S/N=10);在2.0～100.0μg/L范围内阿维菌素的峰强度与质量浓度的线性关系良好(r=0.9994)。3个添加水平(1.0,10和100μg/kg)的平均回收率在79.2%～96.1%,相对标准偏差3.18%～9.14%。田间测定试验结果表明:阿维菌素在苹果中的原始沉积量为11.8～18.4μg/kg,在土壤中的原始沉积量为44.7～54.8μg/kg,代谢速率快,在苹果中的半衰期为2.35～3.84d,在土壤中的半衰期为4.58～6.45d。
A UPLC-MS/MS method was developed to determine and explore the degradation metabolism of abamectin residues in the apples and soils in the apples and soils in different cities including Tai＇an,Xi＇an and Beijing.The samples were extracted with acetonitrile,layered with saturated NaCl salt,and cleaned with NH2-column.The detection was performed by tandem mass spectrometry with electrospray ionization in positive mode （ESI＋ ） .The minimum detectable concentration was 1.0 μg/kg （S/N=10） in samples.A good linear relationship was obtained in the range of 2.0-100.0 μg/L with the correlation coefficient （r） over 0.99.The average recoveries were 79.2%-96.0% spiked at 1.0,10,100 μg/kg.The field results indicated that the original residues of abamectin in apples and in soil were 11.8-18.4 μg/kg and 44.7-54.8 μg/kg,respectively.The half-life of abamectin in apples and in soil was 2.35-3.84 d and 4.58-6.45 d,respectively.The metabolism of abamectin in apples was faster than that in soil.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第10期1505-1509,共5页
Chinese Journal of Analytical Chemistry
基金
山东省科技专项(No.2006GG1108097-34)
山东省农科院博士基金(No.2006YBS019)资助项目
关键词
阿维菌素
液相色谱-串联质谱
苹果
土壤
残留
消解
Abamectin
Ultra performance liquid chromatography-tandem mass spectrometry
Apples
Soil
Residue
Degradation
