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焦磷酸测序技术分析单核苷酸多态性在法医学中的应用 被引量：3

SNP analysis using pyrosequencing and its forensic application
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摘要 单核苷酸多态性(SNP)是新一代的法医学遗传标记,有望成为法医实践中解决高度降解检材及特殊案件DNA鉴定的重要工具。近年来涌现出许多高通量的SNP分析方法,如引物延伸结合时间飞行质谱分析法、微测序法、SNP lex以及焦磷酸测序法等。本文重点对焦磷酸测序技术的原理、步骤及其在法医学中的应用进展进行简要的综述。 Single nucleotide polymorphism （SNP） is a new generation of genetic markers in forensic science, and is expected to be used in DNA typing of highly degraded forensic samples or solving some special DNA analysis. Many high-throughput SNP analysis techniques have emerged in recent years,including primer extension combined matrix assisted laser desorption ionization time of flight mass spectrometry. （ MALDI- TOF-MS）, minisequencing, SNPlex and pyrosequencing method, etc. This article focuses on the principle and steps of pyrosequencing,as well as its application in forensic medicine.
作者 冯婷 李淑瑾 丛斌 娄春光
机构地区 河北医科大学法医学系河北省法医学重点实验室
出处 《中国法医学杂志》 CSCD 北大核心 2010年第5期335-337,341,共4页 Chinese Journal of Forensic Medicine
基金 河北省自然科学基金(C2010000459)
关键词 法医物证学 单核苷酸多态性 焦磷酸测序技术 forensic biological evidence single nucleotide polymorphism pyroseqnencing
分类号 D919 [医药卫生—法医学]
  • 相关文献

参考文献19

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二级参考文献38

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共引文献232

同被引文献49

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引证文献3

二级引证文献24

中国法医学杂志
2010年 第5期

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